...Jon D. Blumenfeld; Jeffrey Tepler; Andreas Mauer; Barry Coller; Daniel G. Bichet; Barry Smith National Institutes of Health dDAVP infusion, before treatment with tolvaptan ( Table 1 ), caused 2- to 3-fold increases in VWF antigen, ristocetin cofactor activity, and factor VIII coagulant...

...Lorena Buitrago; Samuel Lefkowitz; Ohad Bentur; Julio Padovan; Barry Coller The molecular basis of platelet-fibrin interactions remains poorly understood despite the predominance of fibrin in thrombi. We have studied the interaction of platelets with polymerizing fibrin by adding thrombin to washed...

...Lorena Buitrago; Miriam-Rose Menezes; Chloe Larson; Jihong Li; Thomas Kartika; Priyam Banerjee; Fraser Glickman; Barry S. Coller Platelet clot retraction, the ultimate phase of platelet thrombus formation, is critical for clot stabilization. It requires functional αIIbβ3 receptors, fibrin...

...Thomas Kartika; Claudia Lorena Buitrago; Charlotte Cunningham-Rundles; Barry S. Coller Background: Bruton's Tyrosine Kinase inhibitors (BTKi) have transformed the treatment of chronic lymphocytic leukemia (CLL). Paradoxically, while increased bleeding has been reported with BTKi therapy, patients...

...Lu Wang; Jihong Li; Barry S. Coller Introduction: Structure/function studies have shown 3 major conformations of αIIbβ3, bent-closed, extended-closed, and extended-open. We recently noted the interaction of K514 in the thigh domain of αIIb with E500 in the I-EGF2 domain of β3 in the bent, closed...

...Lu Wang; Jialing Wang; Jihong Li; Thomas Walz; Barry S. Coller Monoclonal antibodies (mAbs) have provided valuable information regarding the structure and function of platelet αIIbβ3. Protein disulfide isomerase (PDI) has been implicated in αIIbβ3 activation and binds to thrombin-activated αIIbβ3...

...Lu Wang; Jialing Wang; Jihong Li; Thomas Walz; Barry S. Coller L.W. and J.W. contributed equally Introduction: The platelet integrin αIIbβ3 plays a pivotal role in both hemostasis and thrombosis, and is an established target for antiplatelet therapy. Upon activation, αIIbβ3 undergoes...

...Dragana Nešić; Martin Bush; Aleksandar Spasic; Jihong Li; Tetsuji Kamata; Makoto Handa; Marta Filizola; Thomas Walz; Barry S. Coller The murine monoclonal antibody (mAb) PT25-2 induces αIIbβ3 to bind ligand and initiate platelet aggregation. The underlying mechanism is unclear, because previous...

...Lorena Buitrago; Hina Zafar; Yixiao Zhang; Jihong Li; Thomas Walz; Barry S. Coller Although much is known about the interaction of fibrinogen with αIIbβ3, much less is known about the interaction of platelets with cross-linked fibrin. Fibrinogen residue Lys406 plays a vital role in the interaction...

...Ohad S. Bentur, MD; Barry S. Coller, MD Introduction: RUC-4 is a novel platelet integrin αIIbβ3 antagonist specifically designed for first point-of-medical contact (ambulance or ER) treatment of patients with ST segment elevation myocardial infarction in combination with aspirin. Ongoing Phase 1...

...Dragana Nesic, PhD; Yixiao Zhang, PhD; Aleksandar Spasic, PhD; Jihong Li, MD; Davide Provasi, PhD; Marta Filizola, PhD; Thomas Walz, PhD; Barry S. Coller, MD DN, YZ, AS, & JL contributed equally, as did MF, TW, & BSC The αIIbβ3 antagonist antiplatelet drug abciximab, approved in 1994...

...Claudia Lorena Buitrago, PhD; Barry S. Coller, MD; Hina Zafar The formation of a stable platelet thrombus in vivo is accompanied by the generation of thrombin and the conversion of fibrinogen to cross-linked fibrin. This requires thrombin's action on fibrinogen, producing fibrin monomers...

...Hina Zafar, MS; Jihong Li, MD; Claudia Buitrago, PhD; Dragana Nesic, PhD; Barry S. Coller, MD Background: The interaction between the fibrinogen (fbg) γ-chain sequence 404-411 and the 'RGD pocket' formed by αIIb and β3 plays a major role in platelet (P) aggregation. It requires activation of αIIbβ3...

...Spandana Vootukuri, MS; Jihong Li, MD; Mark Nedelman, MS, MBA; Mariana Babayeva, MD, PhD; Barry S. Coller, MD We are developing the novel αIIbβ3 pure antagonist, RUC-4, for first point of contact treatment of ST Segment Elevated Myocardial Infarction (STEMI) in combination with aspirin. We have...

... Filizola, PhD; Michael Foley, PhD; Barry S. Coller, MD # Contributed equally αVβ3 has been implicated in the pathophysiology of important disorders, including osteoporosis, sickle cell disease, tumor angiogenesis, metastasis, virus invasion, glomerulonephritis, dermal and hepatic fibrosis, acute...

...Barry S. Coller, MD; Claudia Buitrago, PhD Platelet integrin αIIbβ3, glycoprotein-Ib (GPIb), and integrin α2β1 bind extracellular ligands fibrinogen, von Willebrand Factor (vWF), and collagen, respectively. These liganded receptors, in turn, connect the extracellular matrix to the cytoskeleton via...

...Jordan Spradbrow, BSc; Sasha Letourneau; Julie Grabell, CCRP; Yupu Liang, PhD; James Riddel, RN, MS, CPNP; Wilma Hopman, MSc; Victor S. Blanchette, MD; Margaret L. Rand, PhD; Barry S. Coller, MD; Andrew D. Paterson, MD; Paula D. James, MD Introduction: Over the past decade there has been...

...Hina Zafar; Yi Shang; Jihong Li; George A. David, III; Joseph P. Fernandez; Henrik Molina; Marta Filizola; Barry S. Coller Platelet integrin receptor αIIbβ3 supports platelet aggregation by binding fibrinogen. The interaction between the fibrinogen C-terminal γ-chain peptide composed of residues γ...

... ofmonoclonal anti-platelet GP lb antibody on the TTPplasma-induced platelet aggregation. A 1.5- tL of buffer or monoclonal anti-platelet GP lb antibody solutions (which was kindly supplied by Dr Barry Coller, SUNY, Stony Brook, NY, and designated 6DI) containing 0.67 g protein was mixed with 0.1 mL ofwashed...

...Barry S. Coller This year we celebrate Blood 's 70th year of publication. Created from the partnership of the book publisher Henry M. Stratton and the prominent hematologist Dr William Dameshek of Tufts School of Medicine, Blood has published many papers describing major advances in the science...