BERNARD W. AGRANOFF, BERT L. VALLEE, DAVID F. WAUGH; Centrifugal Subfractionation of Polymorphonuclear Leukocytes, Lymphocytes and Erythrocytes. Blood 1954; 9 (8): 804–809. doi: https://doi.org/10.1182/blood.V9.8.804.804
The separations of polymorphonuclear leukocytes from lymphocytes and erythrocytes has been accomplished by employing a method which takes advantage of their density differentials. During a single centrifugations the lymphocytes accumulate at a plasma-albumin interface (albumin I, density = 1.065 to 1.085), the polymorphonuclear leukocytes accumulate at an albumin I-albumin II interface (albumin II, density = 1.094) and the erythrocytes pass to the base of the Wintrobe tube. The yields are over 90 per cent pure cell types. All layers are isosmotic with blood.