A1, a bcl-2 family member, has been identified as a hematopoietic- specific, early inducible gene. In this study it is shown that stable transfection of A1 into an interleukin-3 (IL-3)-dependent myeloid precursor cell line, 32D c13, leads to a retardation of IL-3 withdrawal- induced cell death similar to that observed with transfection of bcl-2. However, unlike bcl-2. A1 expression permits the accumulation of differentiated myeloid cells both before and after IL-3 withdrawal. Total cell accumulation, on the other hand, is considerably greater after IL-3 deprivation in the bcl-2 transfectant than in A1-expressing cells. Cells cotransfected with the two genes behave similarly to cells singly transfected with bcl-2, except that viability following IL-3 withdrawal is somewhat further enhanced. These results suggest that these two proteins have distinct roles that may be related to the divergent regulation of their expression during myeloid differentiation.
A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation
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EY Lin, A Orlofsky, HG Wang, JC Reed, MB Prystowsky; A1, a Bcl-2 family member, prolongs cell survival and permits myeloid differentiation. Blood 1996; 87 (3): 983–992. doi: https://doi.org/10.1182/blood.V87.3.983.bloodjournal873983
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