The occurrence of a potent antibody against plasmatic A and B glycosyltransferase activities has been characterized in a patient (blood group A1) transplanted with a bone marrow from a blood group O donor. A and B glycosyltransferases were purified to near homogeneity from plasma of A1 and B blood-group individuals. The half-maximal inhibition of both enzymes was obtained at 1 to 2 micrograms/mL of the post-transplant IgG fraction, prepared by protein A-sepharose chromatography. A and B glycosyltransferases were also recognized by the post-transplant IgG fraction after sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) followed by electrophoretic transfer to nitrocellulose membranes.
Identification of an anti-A and anti-B blood group glycosyltransferase antibody after incompatible bone marrow transplant
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M Mojena, L Bosca; Identification of an anti-A and anti-B blood group glycosyltransferase antibody after incompatible bone marrow transplant. Blood 1989; 74 (3): 1134–1138. doi: https://doi.org/10.1182/blood.V74.3.1134.bloodjournal7431134
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