Anti-murine (m) interleukin-5 (IL-5) antibody was found to inhibit eosinophil (Eo) colony formation stimulated by recombinant human (rh) IL-5, but did not inhibit the production of Eo stimulated by rh IL-3 or granulocyte-macrophage colony-stimulating factor (GM-CSF). Conditioned medium (CM) prepared from eosinophilic patients' T cells with interleukin-2 (IL-2) stimulation (T-IL-2-CM), was found to contain CFU- Eo growth-stimulating factor. Using anti-mIL-5 antibody, we demonstrated that T-IL-2-CM from patients with eosinophilia contained a significant amount of IL-5. We also detected IL-5 mRNA in T cells from eosinophilic patients with IL-2 stimulation. These results suggest that IL-5 plays an important role in the induction of selective eosinophilia in humans and that IL-5 is produced from T cells with IL-2 stimulation.
T cells from eosinophilic patients produce interleukin-5 with interleukin-2 stimulation
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H Enokihara, S Furusawa, H Nakakubo, H Kajitani, S Nagashima, K Saito, H Shishido, Y Hitoshi, K Takatsu, T Noma; T cells from eosinophilic patients produce interleukin-5 with interleukin-2 stimulation. Blood 1989; 73 (7): 1809–1813. doi: https://doi.org/10.1182/blood.V73.7.1809.bloodjournal7371809
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