Blood from six donors was stored in two experimental blood preservatives, CPD-adenine-sodium ascorbate and bicarbonate-adenine-glucose-phosphate-mannitol. Control aliquots were stored in CPD-adenine and ACD-adenine preservatives. Half of each donor’s blood was stored without mixing and half was mixed vigororously 5 days each week. Glucose, ATP, and 2,3-DPG levels were measured at various times during storage in both the mixed and unmixed bags. Mixed bags maintained higher levels of glucose, ATP, and 2,3-DPG throughout storage. Frequent mixing was shown to be essential for the maintenance of 2,3-DPG with these preservatives.
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