A method is described by means of which the rapidly denatured adult Hb can be separated from the slowly denatured fetal Hb by denaturing with alkali and precipitating the denatured material at its isoelectric point. When applied to 15 normal cord bloods and to the cord bloods of 15 infants affected with hemolytic disease of the newborn, the method showed no significant difference in the percentage of fetal Hb present.

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