By applying Schiff’s reagent after periodic acid treatment to blood and bone marrow films, a cytoplasmic staining reaction is seen in some cells of the myeloid series, as well as in megakaryocytes and platelets. The intensity of the staining reaction in the myeloid cells increases with their maturation. The staining reaction can be prevented altogether in alcohol-fixed films by salivary digestion, but only incompletely in air-dried films. The staining reaction is due to the presence of glycogen in some chemical association, possibly with protein.

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