Red cells were depleted of 2,3-diphosphoglycerate (2,3-DPG) by storage in acid-citrate-dextrose (ACD) solution. No change in the sickling-oxygen saturation curve was observed. 2,3-DPG did not affect the viscosity of deoxygenated sickle hemoglobin solutions. It appears, then, that the interaction between 2,3-DPG and sickle hemoglobin does not produce configuration changes which influence the aggregation leading to sickling.

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