A rapid spot screening test for NADH-diaphorase deficiency is described. Nitrited blood is added to a reaction mixture freshly prepared from stable stock reagents. Spots are made at intervals on filter paper and examined for defluorescence as NADH is oxidized. All of six patients with NADH diaphorase deficiency studied have been correctly identified using this technique, but it is not suitable for the detection of heterozygotes.

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