Purification without Loss of Viability of Neoplastic Plasma-Cells from the Ascites Passaged Mouse Myeloma, X5563

A simple scheme for purification of murine myeloma cells from ascites fluid without loss of viability is presented. This is accomplished by repeated differential centrifugations followed by passage through a glass bead column. The cells so obtained are 99.9 per cent pure and 99 per cent viable in a large number of repetitions of this technic.