Stoward1 conjugated acidified solutions of salicyloyl hydrazide with the dialdehydes formed from the periodic acid oxidation of vicinal glycols in guinea pig tissue sections. The method has now been utilized, with minor modification, to demonstrate glycogen in blood and marrow cells, and it has been compared with the periodic acid-Schiff reaction and with a fluorescent acriflavine Schiff-type method. It is felt that the PA-SH method will replace the existing Schiff-type fluorescent methods and that it will prove to be a useful technic to aid in the diagnosis of blood conditions, such as acute leukemia, where PAS positivity is known to occur.

This content is only available as a PDF.