Preservation of a graft of lymph node cells or semi-allogenic bone marrow cells at 37 C. in Tyrode’s solution for 2 hours, which reduces the percentage of cells not permeable to eosin by half, has a statistically significant reducing effect on the frequency of acute or chronic secondary syndromes which occur in irradiated recipients. Preservation at 18 C. for 6 hours, which in the same way increases the percentage of cells permeable to eosin, does not have the same effect.

These two methods of preserving bone marrow cells do not appreciably reduce the myeloid-restoring capacity of compatible or incompatible irradiated recipients.

Application of these results to bone marrow grafting in clinical medicine is discussed.

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