A method for measurement of radioactive hemin formation has been applied to the in vitro system for culture of human erythroblasts previously described. The results of these measurements have been shown to correlate with a morphologic parameter of maturation, the increase of orthochromic cells, in the same in vitro cell culture system.

The significance of these findings as well as the differences in the times of formation of heme and the increase of orthochromic cells has been discussed.

It is proposed that both the formation of radioactive hemin and the increase of orthochromic cells may be useful in the measurement of different aspects of the effects of various plasmas on the maturation of normal and abnormal human erythroblasts.

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