During late erythropoiesis, exogenous glutamine, rather than TCA cycle intermediates, provides carbons for succinyl-CoA for heme synthesis.
Itaconate, a compound manufactured as part of the inflammatory response, inhibits heme synthesis in cells undergoing erythropoiesis.
During erythroid differentiation, the erythron must remodel its protein constituents so that the mature red cell contains hemoglobin as the chief cytoplasmic protein component. For this, ∼109 molecules of heme must be synthesized, consuming 1010 molecules of succinyl-CoA. It has long been assumed that the source of succinyl-coenzyme A (CoA) for heme synthesis in all cell types is the tricarboxylic acid (TCA) cycle. Based upon the observation that 1 subunit of succinyl-CoA synthetase (SCS) physically interacts with the first enzyme of heme synthesis (5-aminolevulinate synthase 2, ALAS2) in erythroid cells, it has been posited that succinyl-CoA for ALA synthesis is provided by the adenosine triphosphate–dependent reverse SCS reaction. We have now demonstrated that this is not the manner by which developing erythroid cells provide succinyl-CoA for ALA synthesis. Instead, during late stages of erythropoiesis, cellular metabolism is remodeled so that glutamine is the precursor for ALA following deamination to α-ketoglutarate and conversion to succinyl-CoA by α-ketoglutarate dehydrogenase (KDH) without equilibration or passage through the TCA cycle. This may be facilitated by a direct interaction between ALAS2 and KDH. Succinate is not an effective precursor for heme, indicating that the SCS reverse reaction does not play a role in providing succinyl-CoA for heme synthesis. Inhibition of succinate dehydrogenase by itaconate, which has been shown in macrophages to dramatically increase the concentration of intracellular succinate, does not stimulate heme synthesis as might be anticipated, but actually inhibits hemoglobinization during late erythropoiesis.
The ability of a cell to maintain appropriate levels of key metabolites in response to changing organismal and environmental demands is essential for survival and growth. Monitoring and understanding regulatory schemes is challenging, but essential if one is to understand the metabolic basis for diseases as well as the potential impact of xenobiotics on cellular metabolism. All cell types have their own metabolic and regulatory needs, but those undergoing differentiation into a new cell type present unique challenges. This is clearly true for cells undergoing erythropoiesis where, starting with hematopoietic stem cells (HSCs), every cell division results in daughter cells that are distinct from their parent.1,2 Adding complexity to the overall process is that erythropoiesis occurs within the bone marrow in erythroblastic islands where red cell precursors exist in close association with so-called nurse macrophages.3,4
One of the hallmark events of terminal erythroid differentiation is the coordinated induction of heme and globin synthesis. Onset of heme synthesis occurs when the first pathway enzyme, the erythroid-specific 5-aminolevulinate synthase (ALAS2), is induced.5 In humans, this occurs in the basophilic erythroblast, reaching a maximum in the polychromatic/orthochromatic erythroblast, whereas in murine systems the initial induction occurs earlier, in the proerythroblast.6 GATA1-induced erythroid differentiation results in the upregulation of heme synthesis by induction of ALAS2, enhancement of the transcription of all heme pathway enzyme genes,5-7 and phosphorylation of the terminal pathway enzyme, ferrochelatase (FECH).8 In addition to transcriptional regulation, ALAS2 is also subject to translational regulation by iron via the iron-regulatory protein/iron-responsive element machinery9-12 and microRNAs.13 Posttranslational modification of the protein via the addition of the cofactor pyridoxal phosphate is essential.14 Hydroxylation of ALAS2 decreases the activity of ALAS2 and destabilizes it for proteasomal degradation,15 and posttranslational translocation of ALAS into the mitochondrion is inhibited by the binding of heme to the cytoplasmic precursor form of ALAS.16-18
Synthesis of heme in metazoan cells requires iron, glycine, and succinyl-coenzyme A (CoA) (supplemental Figure 1, available on the Blood Web site).2,19 The supply of iron for heme both in housekeeping activities and during erythroid differentiation has been well studied, and a reasonable view of the components involved in this process at both the organismal and the cellular level exists.20 Glycine is abundant in plasma (∼250 µM), and studies of glycine transporters are consistent with glycine being supplied from extracellular sources.21-23 In contrast, our knowledge of how succinyl-CoA is provided for heme synthesis during erythroid differentiation is not well understood. The assumption that the tricarboxylic acid (TCA) cycle supplies succinyl-CoA for erythroid heme synthesis has been made, but there is a paucity of data to support this view. It is possible that for nonerythroid cells, where only modest levels of heme are synthesized, there is sufficient capacity from the TCA cycle to supply succinyl-CoA.24 However, evoking this mechanism for erythroid cells would clearly place cells under strain as every molecule of heme generated requires 8 molecules of succinyl-CoA, and cells make 1.2 × 109 molecules of heme in a span of 2 to 3 days. It has been shown that ALAS2, but not the housekeeping ALAS (ALAS1), physically interacts with the adenosine triphosphate (ATP)-dependent succinyl-CoA synthetase (SCS) β-subunit SUCLA2.25,26 Because ALAS2 utilizes succinyl-CoA to synthesize ALA, and because SUCLA2 as a subunit of SCS may be involved in the ATP-dependent reverse reaction of SCS to generate succinyl-CoA from succinate, one hypothesis forwarded was that the ALAS2-SUCLA2 interaction exists to provide succinyl-CoA for ALA synthesis during erythropoiesis. However, although it is clear that an association between ALAS2 and SUCLA2 can exist, there are no data available to demonstrate what role, if any, this interaction plays in the supply of succinyl-CoA for ALAS2.
Data gathered in the current work from cells undergoing erythroid differentiation in vitro demonstrate that succinyl-CoA for ALA synthesis does not arise from succinate of the TCA cycle, but from α-ketoglutarate (KG) derived from glutamine and that during late erythropoiesis cellular metabolism is remodeled so that the major role of glutamine is to serve as a precursor for heme synthesis. This has significant implications to our understanding of cellular metabolism during erythropoiesis. Interestingly, we found that itaconate, a compound produced and excreted by lipopolysaccharide-induced macrophages,27 that is known to cause an increase in cellular succinate levels by inhibiting succinate dehydrogenase (SDH),27-29 inhibits heme synthesis and erythroid differentiation and that this inhibition is reversed by exogenously supplied ALA. A physiological role for macrophage-generated itaconate during erythropoiesis has not been identified, but data presented here suggest that it inhibits ALA synthesis in an as yet unidentified fashion.
13C labeling, cellular extraction, and liquid chromatography–mass spectrometry (LC/MS) characterization of murine erythroleukemia (MEL) cells undergoing erythroid differentiation are described in the supplemental materials. Assays of TCA cycle enzymes were done with mitochondria isolated from differentiating MEL cells as detailed in the supplemental materials. CD34+ cells were expanded and differentiated following StemCell protocols (StemCell Technologies Inc). Additional details are in the supplemental materials. Flow cytometry was carried out at the University of Utah Flow Cytometry Facility.
Fate of 13C-labeled metabolic heme precursors during erythroid differentiation
Protoheme IX has a total of 34 carbons with 26 of these derived from succinyl-CoA and 8 carbons derived from glycine (Figure 1B-C). Assuming that glycine for heme synthesis is exogenously supplied,22,23 then 76% of the heme carbons originate from a source that produces succinyl-CoA (Figure 1A). During late erythropoiesis, elevation of heme synthesis creates a significantly increased demand for succinyl-CoA believed to come from the TCA cycle. Draining this cycle intermediate would require replenishment via anaplerotic supplementation.30 Both glucose and glutamine can replenish the TCA cycle by the conversion of pyruvate into oxaloacetate and glutamate into α-KG, respectively. In order to determine if either of these anaplerotic reactions provides carbon for heme synthesis during erythropoiesis, we employed uniformly (U) labeled 13C precursors and followed their incorporation into heme in differentiating MEL cells via LC/MS. MEL cells were induced to differentiate, and for a 24-hour period between 48 and 72 hours postinduction, the time of maximal heme synthesis in this system, the medium was supplemented with U-13C-labeled (1) glucose, (2) glutamine, or (3) diethylsuccinate (DES), the commonly employed cell-permeable analog of succinate.31 Heme was extracted and isolated from cells at 72-hours postinduction prior to analysis by LC/MS. A 24-hour window of exposure to U-13C-labeled metabolite was chosen in an effort to minimize cellular reshuffling of the label.
The data obtained revealed that 2.5% of heme carbons were labeled when U-13C DES was the sole source of isotope label, whereas 8.3% were labeled with U-13C glucose and 23% of heme carbons were labeled by U-13C glutamine. Because the amount of heme synthesized in the first 48 hours postinduction is not subject to labeling, parallel experiments where heme synthesis was inhibited at 48 hours by the aminolevulinate dehydratase (porphobilinogen synthase) inhibitor succinylacetone32 were carried out. These demonstrated that 30% of the heme present at 72 hours was synthesized prior to the 24-hour period of the 13C-labeling experiments. Thus, total U-13C-labeled DES, glucose, and glutamine contributions are 3.6, 12.0, and 33.0%, respectively.
Isotopomer data demonstrated that succinate supplied as U-13C DES provided significant label in protoporphyrin only up to a total of 5 carbons (M+5) with the greatest contribution above background level to be M+2, M+3, and M+4 (Figure 2A). These data points indicate that succinate is not directly converted to succinyl-CoA by reverse SCS and that it is also a poor source of carbon for ALAS2 even after a passage through the TCA cycle. U-13C from glucose had the highest degree of labeling around M+8 and provided labeling above background approaching M+16 (Figure 2B). Significant labeling was seen as low as M+2 but not as high as M+18. These data points demonstrate that the overall contribution of glucose to cellular heme synthesis during erythropoiesis (12%) is minor. Glutamine-supplied U-13C resulted in heme with a maximum incorporation around M+13 and significant label up to M+23 (Figure 2C). There is no significant labeling below M+4. These data points show that glutamine is a superior precursor of succinyl-CoA for ALAS2 than is glucose or succinate and that it is used directly rather than passing through the TCA cycle (see supplemental materials). The isotopomer data suggest that no single compound in the cell during erythropoiesis is the sole source of carbons for the succinyl-CoA that is used by ALAS2. However, it is clear that glutamine is the most effective and plays a significant role during elevated heme synthesis.
Realizing that glucose may be metabolized to glutamate and that this may cause an apparent dilution of glutamine label going into heme during erythropoiesis, we examined the amount of glutamine and glutamate in the cell that were produced from U-13C labeled glucose during the experimental period (Figure 2D). The data indicate that glucose contributes ∼10% to the cellular glutamate and glutamine pools at the M+2 level even when medium-supplied glutamine is present. Presumably this results from transamination of TCA cycle-derived α-KG. Thus, the isotopomer-based data for heme carbons derived from glutamine represent an underestimation, because the available U-13C glutamine pool is significantly diluted by endogenous unlabeled glutamine.
As shown in Figure 2E, in differentiating MEL cells, glucose is converted into the TCA cycle intermediates fumarate and malate as the M+2 and M+3 isotopomers better than is glutamine. This is consistent with our model, where glutamine is preferentially converted into succinyl-CoA for use by ALAS2 and not for a generic replenishing of the TCA cycle. However, glutamate is the precursor to proline, and this is evident from 13C- and 15N-glutamine labeling of proline in differentiating MEL cells (Figure 2F). Not surprising, and in accord with our data presented above, glucose also contributes carbons to proline via TCA cycle-generated α-KG that is transaminated to glutamate for subsequent proline synthesis.
TCA cycle enzymes during erythropoiesis
If alterations in central metabolite flux occur upon initiation of erythropoiesis, one might expect a corresponding change in key enzyme activities. Thus, if succinyl-CoA for ALA synthesis is supplied by the TCA cycle, there would be an increase in TCA cycle enzymes. In contrast, for a model that has glutamine as the major source of carbon for ALA, it would be anticipated that there would be an increase in the enzyme α-ketoglutarate dehydrogenase (KDH), but not necessarily an increase in all other TCA cycle enzymes. KDH is composed of 3 types of subunits: oxoglutarate dehydrogenase (E1), dihydrolipoamide S-succinyltransferase (E2), and dihydrolipoamide dehydrogenase (E3).33,34 Examination of data in ErythronDB35,36 reveals that during adult definitive erythropoiesis messenger RNA (mRNA) levels for the KDH subunit E1 have a positive correlation with heme synthesis enzymes ALAS2 (0.92) and FECH (0.90), which both show significant increases during erythropoiesis. However, ALAS2 and FECH have negative correlations with many TCA cycle enzymes (SDHB, −0.49; SUCLA2, −0.55; ACLY, −0.57; and SDHD, −0.75), which generally remain constant or decrease. Realizing that mRNA levels may not always accurately reflect enzyme activities, we measured the activity of 6 TCA cycle enzymes in MEL cells (Figure 3A).
Only aconitase, isocitrate dehydrogenase, and KDH showed increases in cells undergoing erythroid differentiation relative to their activity in undifferentiated cells. Due to the significant increase in KDH enzyme activity as measured in vitro, further examination of KDH was warranted because it plays a central role in any model of heme synthesis. We verified that the ErythronDB mRNA increase of E1 was reflected in protein expression in differentiating MEL cells via western blot analysis (Figure 3B). There is a more than threefold increase in E1 protein. Thus, mRNA, protein, and enzyme activity measurements all demonstrate an increase in the E1 KDH subunit during erythropoiesis.
Because KDH can be a nucleating point for some TCA cycle enzymes,33 we wondered if it might interact with ALAS2. When purified, His-tagged, recombinant ALAS2 and untagged KDH purified from porcine hearts were mixed in solution and applied to a cobalt resin column, and the E1 component of KDH coeluted with ALAS2 (Figure 3C). This illustrates that in vitro the E1 component of KDH interacts with ALAS2.
In addition, in situ interactions between KDH and ALAS2 were probed with antibody pull-downs of mitochondria from differentiating MEL cells using flag-tagged ALAS2 as bait as described previously.37 These data points clearly demonstrated that ALAS2 interacts with the E1, E2, and E3 subunits of KDH (Table 1).
|Murine protein .|
|Human input .||Kdh E1 .||Kdh E2 .||Kdh E3 .||Alas2 .||Fumarate hydratase .|
|ALAS2 (n = 2)||20-45||10-23||10-20||90-347||4-10|
|Empty vector (n = 2)||0-2||6-7||0-4||0||3-6|
|Murine protein .|
|Human input .||Kdh E1 .||Kdh E2 .||Kdh E3 .||Alas2 .||Fumarate hydratase .|
|ALAS2 (n = 2)||20-45||10-23||10-20||90-347||4-10|
|Empty vector (n = 2)||0-2||6-7||0-4||0||3-6|
Data shown top to bottom as spectral counts, number of unique peptides, and percent coverage, respectively.58
Impact of itaconate
Factors that influence the cellular supply of succinyl-CoA during erythropoiesis could clearly have an impact on heme synthesis. Itaconate, which is produced from the TCA cycle intermediate cis-aconitate by the enzyme IRG1, has been shown to disrupt the TCA cycle by blocking SDH resulting in increased cellular succinate.27-29,38-40 Given that itaconate causes accumulation of intracellular succinate, it could be imagined that it might stimulate porphyrin synthesis by increasing the amount of succinyl-CoA available (from reverse ATP-dependent SCS activity). In differentiating MEL cells, we monitored heme synthesis during erythroid differentiation41 (Figure 3D). Our data demonstrated that addition of 2.5 mM itaconate to cultures of differentiating cells did not result in an increase in heme synthesis, but instead caused an arrest in hemoglobinization. Significantly, this block could be reversed by adding ALA to the culture. These results are consistent with the finding from the isotopomeric studies that succinate is not the main source of succinyl-CoA for heme synthesis during late erythropoiesis.
Impact of metabolic inhibitors on erythroid heme synthesis
In order to further probe the source of precursors of α-KG and succinyl-CoA for heme synthesis, the impact of inhibitors that target enzymes potentially required for the synthesis of succinyl-CoA was examined in cells undergoing erythropoiesis. Aminooxyacetic acid (AOA), a broad-spectrum inhibitor of pyridoxal phosphate–dependent transaminases, has been shown to block early erythroid differentiation of CD34+ HSCs.42 AOA blocks glutamine deamination and thus the eventual conversion of glutamine into α-KG.43 This block can be bypassed by the addition of dimethyl-α-ketoglutarate (DMK), a cell-permeable analog of α-KG, to culture medium. Given that our data support the hypothesis that glutamine serves as source of carbons for heme synthesis by way of α-KG, we tested whether AOA impairs late erythroid differentiation by blocking heme synthesis. To do this, we examined the level of heme (as hemoglobin) produced by differentiating MEL cells treated with AOA.
MEL cells were selected because they are transformed murine spleen red cell precursors that are arrested at the proerythroblast stage. Upon DMSO-induced differentiation, there is an induction of heme biosynthesis.44,45 Addition of AOA at the initiation of erythroid differentiation reduced hemoglobin content by 76% as compared with untreated cells (Figure 4A). The addition of either DMK or ALA to AOA-treated differentiating MEL cell cultures rescued hemoglobinization (Figure 4B), whereas supplementing cultures with DES did not. These data points are consistent with the proposal that succinyl-CoA for heme synthesis during erythropoiesis arises from α-KG, and not succinate from the TCA cycle.
In addition to MEL cells, we also examined human CD34+ cells to differentiate between cellular needs for glutamine prior to and during hemoglobinization. Human CD34+ cells are primary cells that represent a population of HSCs. When induced toward erythroid differentiation, they first pass through several early stages of erythropoiesis before becoming heme synthesizing basophilic erythroblasts.46 We monitored early erythroid differentiation at day 6, prior to significant elevation of ALAS2 and FECH that occurs at day 7 (supplemental Figure 2), by following expression of the surface receptors CD36, CD71 (transferrin), and CD235a (glycophorin A) and determined the impact that addition of DMK, DES, nucleosides, and/or ALA had on the differentiating CD34+ cells (Figure 5A). As previously reported,42 we found that the AOA-inhibited early erythroid differentiation could be overcome by addition of DMK to the medium (Figure 5B). This inhibition was not overcome by individual additions of DES, ALA, or nucleosides. These data points are consistent with the acknowledged multiple roles of glutamine (via α-KG) in cells undergoing the early stages of erythropoiesis.
For cells that are undergoing late erythropoiesis and beginning to hemoglobinize, we measured cellular heme content41 at days 3 and 6 following removal of cytokines/growth factors other than erythropoietin (EPO). For these cells, we found that AOA inhibited hemoglobinization and that this could be overcome by addition of DMK and ALA, but not DES, to the medium (Figure 5C; supplemental Figure 3). These data points indicate that a major role served by glutamine during hemoglobinization must be for heme synthesis because ALA alone overcame the AOA block during late stages of erythropoiesis. Interestingly, when media were supplemented with ALA, there was background fluorescence, likely due to the presence of unmetallated porphyrin, that was not seen in the DMK or DES supplemented samples.
In going from a proerythroblast to mature erythrocyte, cells can repurpose amino acids from a wide variety of cytoplasmic proteins for synthesis of new globins chains, but each cell must provide ∼1010 molecules of succinyl-CoA and an equivalent amount of glycine for heme synthesis. Although plasma and mitochondrial membrane glycine transporters support the provisioning of glycine from the circulatory system during erythropoiesis,21-23 significant stores of succinyl-CoA do not exist and so this must be provided endogenously in a coordinated, real-time fashion. It is conceivable that in nonerythroid cells the TCA cycle can supply succinyl-CoA without compromising other metabolic pathways. However, given the level of heme synthesis during erythropoiesis, it is implausible that the TCA cycle would be able to function without robust anplerosis to resupply intermediates.
Although there have been proposals for the TCA cycle SUCLA2-dependent reverse SCS supply of succinyl-CoA for ALAS2,25,26 there exist no experimental data to support this hypothesis, and a number of observations are not consistent with this model. First, early studies with the particulate fraction from anemic chicken reticulocytes showed that α-KG served more efficiently than succinate as a precursor for ALA synthesis.19,47 Second, from an overall carbon economy perspective, utilizing TCA cycle intermediates for ALA production would require large-scale replenishment of the TCA cycle via anaplerotic reactions, not to mention the energetic costs of running the SCS reaction backward to generate succinyl-CoA for heme. Third, mice heterozygous for SUCLA2 knockout48 and human patients with SUCLA2 deficiency (MIM ID #612073) exhibit no signs of anemia, which one would anticipate if the ALAS2-SUCLA2 interaction is essential for the supply of succinyl-CoA for heme synthesis. Fourth, mRNA expression profiling during erythropoiesis shows a negative correlation (−0.7) between SUCLA2 and ALAS2 gene expression.35,36 Fifth, ALAS2 variants that have decreased interactions with SUCLA2 do not exhibit a uniform impact on ALAS2 activity. Some mutations result in diminished ALAS2 activity causing sideroblastic anemia,25,26 whereas others result in increased ALAS2 activity causing X-linked protoporphyria.49 Finally, we have demonstrated that succinate provided to differentiating erythroid precursor cells is a poor source of carbons for ALA synthesis when compared with glutamine.
What role SUCLA2 plays is unclear at present. Because it binds to both ALAS2 and FECH,37 it could be that during early stages of differentiation it stabilizes the apoprotein forms of ALAS2, which requires pyridoxyl phosphate as a cofactor,50 and FECH, which requires the presence of a [2Fe2S] cluster.51 Another possibility is that by SUCLA2 binding to ALAS2 and FECH it may prevent assembly of functional ATP-dependent SCS and, thereby, reduce conversion of succinyl-CoA into succinate when ALAS2 levels are high.
Our isotopomer-based analysis clearly demonstrated that glutamine is the preferred source of carbon for protoporphyrin synthesis (Figure 2C). Experimentally we determined that 13C-glutamine labeled 30% of the eligible total carbons in heme.
However, liquid LC/MS analysis reveals that only about one-third of the glutamine in the cell after 24 hours of incubation contains 13C label and that glucose contributes ∼10% of the cellular glutamate and glutamine pools. With these factors taken into consideration, we estimate that the experimentally determined magnitude of carbon flux from glutamine into heme is low by at least a factor of 2. A model where ALA is derived from glutamine via KDH rather than from succinate via SCS (Figure 6) is consistent with both the data presented herein and the published clinical observation that deficiency in thiamine, a required cofactor for KDH, results in a sideroblastic anemia52,53 similar to that found in X-linked sideroblastic anemia caused by deficiency in ALAS2 activity.
Our data are consistent with a separation of glutamine for heme synthesis from a general anaplerotic replenishment of the TCA cycle in that we show glucose contributes to the TCA cycle intermediates during erythropoiesis more effectively than glutamine.
A glucose-derived label is found in malate and fumarate at higher levels than glutamine-derived malate and fumarate. This, along with the observation that 13C label from exogenously supplied glucose is found in significant amounts in cellular glutamate (Figure 2D), suggests that glucose may make contributions to heme via the cellular glutamate pool.
One interesting observation was that exposure of differentiating erythroid cells to itaconate, which is produced and secreted by immunoactivated macrophages and has been shown to increase intracellular succinate levels,27,28 did not stimulate heme synthesis as might be anticipated if succinate is a precursor for ALA synthesis. Instead, it inhibited hemoglobinization of these cells. This inhibition was rescued by addition of ALA to the culture medium, demonstrating that the blockage in hemoglobinization caused by itaconate occurs by inhibiting the availability of a necessary precursor or by directly inhibiting ALA production. This observation that itaconate decreases hemoglobinization in the developing erythron may be evidence for a link between anemia of inflammation and heme production. Itaconate is produced from the TCA cycle intermediate aconitate by the enzyme IRG1 and Irg1 as 1 of the most highly upregulated genes in macrophages under proinflammatory conditions28,38 Activated macrophages, which are present in erythroblastic islands along with erythroid precursor cells,54,55 excrete high concentrations of itaconate into the surrounding milieu. Thus, the action of itaconate on heme synthesis bears a resemblance to the action of hepcidin on heme synthesis during chronic infection.56 Both hepcidin and itaconate are antimicrobial compounds produced during the inflammatory response, and both inhibit heme synthesis, hepcidin by regulating iron and itaconate by regulating porphyrin synthesis. Thus, these may represent complementary systems that not only regulate overall heme synthesis but also prevent the accumulation of 2 toxic compounds: protoporphyrin and iron.
Glucose and glutamine metabolism via KDH have been shown to be essential for erythroid lineage commitment of CD34+ HSCs.43 Because glutamine is the most abundant amino acid in plasma, and plasma glucose levels in a healthy person range from 4 to 6 mM, both metabolites are readily available to developing erythroblasts. Knockdown of the glucose transporter Glut1 or the glutamine transporter ASCT2 in CD34+ stem cells decreased erythroid differentiation as measured by erythroid lineage-specific surface antigens. Oburoglu et al42 reported that glutamine is essential for nucleotide biosynthesis during erythropoiesis. Their conclusion that glutamine is required for nucleotide synthesis was based upon nucleoside rescue of CD34+ cells treated with 6-diazo-5-oxo-l-norleucine (DON). DON is a known inhibitor of glutamine utilizing enzymes, such as carbamoyl phosphate synthase, CTP synthase, NAD synthase, guanosine monophosphate synthetase, glutamine phosphoribosylpyrophosphate amidotransferase, formylglycinamide ribonucleotide amidotransferase, and asparagine synthase, which can all directly block nucleotide synthesis.57 This is aside from its ability to function as a glutaminase inhibitor. Because of DON’s targeted role in transaminase-independent inhibition of nucleotide synthesis, we focused on inhibition by AOA, which is more restricted to transaminases and not downstream pathways. Interestingly, we found that AOA inhibition of early erythropoiesis of CD34+ cells was not reversed by addition of nucleosides to the medium. Likewise, unlike DMK, ALA addition either alone or with nucleosides did not rescue the appearance of cell surface markers of erythroid maturation in CD34+ cell during early erythropoiesis. These data points demonstrate that glutamine plays a much greater role during early erythropoiesis than simply as a precursor to heme and nucleotide synthesis. The fact that either DMK or ALA rescues the AOA-mediated block of hemoglobinization in MEL cells and late-stage CD34+ cells supports an essential role for α-KG in hemoglobinization during late erythropoiesis. Glucose, which was present in all culture media, and DES did not rescue hemoglobinization in these cells, supporting the fact that their contributions alone to the production of succinyl-CoA via the TCA cycle are insufficient to support heme synthesis during erythropoiesis. However, it is clear from the 13C labeling, enzyme activity, cell culture data, and identification of the interaction between KDH and ALAS2 that KDH, in addition to being a component of the TCA cycle, serves a critical “moonlighting” role as primary supplier to succinyl-CoA for heme synthesis during erythropoietic hemoglobinization.
The online version of this article contains a data supplement.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 USC section 1734.
The authors gratefully acknowledge Tamara A. Dailey (deceased) for outstanding editorial assistance and critical discussions.
This work was supported by National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases grants DK111653 (A.E.M.), U54 DK110858, DK090257 (J.D.P.), DK096501 (H.A.D.), NIH Office of the Director grant S10 OD016232 (J.C.) and by the University of Utah Flow Cytometry Facility in addition to the National Institutes of Health, National Cancer Institute through Award Number 5P30CA042014-24. Research reported in this publication was supported by the National Center for Research Resources of the National Institutes of Health under Award Number 1S10RR026802-01.
Contribution: J.S.B. designed experiments, analyzed data and wrote the paper; J.R.M., J.A.M., and L.K.J. performed research and analyzed data; J.E.C., A.E.M., and J.D.P. analyzed data and edited the paper; and H.A.D. designed experiments, analyzed data, and wrote the paper.
Conflict-of-interest disclosure: The authors declare no competing financial interests.
Correspondence: Harry A Dailey Jr, Department of Microbiology, 230 Coverdell Center, University of Georgia, Athens, GA 30602; e-mail: email@example.com.