In an effort to isolate human leukocytes as free as possible of erythrocytes, a two-stage method has been studied. The initial step, sedimentation of the erythrocytes with Dextran results in a diminution of RBC:WBC ratio from the 1000:1 of peripheral blood, to 2.34:1. After sedimentation in isotonic saline, the RBC:WBC ratio is decreased to 0.16:1.

This method should prove valuable in preparing leukocytes for immunologic studies.

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