Lower-risk myelodysplastic syndromes (MDSs) are defined as having low or intermediate 1 risk by the International Prognostic Scoring System and are characterized mainly by anemia in most cases. Supportive care—primarily red blood cell transfusions—remains an important component of their treatment, but exposes patients to insufficient correction of anemia, alloimmunization, and organ iron overload (for which the role of iron chelation remains debated). Treatment aimed at preventing anemia recurrence should therefore be used whenever possible. Erythropoiesis stimulating agents remain the first-line treatment of anemia in most lower-risk MDS without del(5q), whereas anemia of low-risk MDS with del 5q responds to lenalidomide in two-thirds of the cases, but this drug should be used cautiously because profound cytopenias may occur initially. Treatment after failure of those first-line therapies are disappointing overall, with many patients eventually requiring long-term transfusions, but encouraging results have been reported with hypomethylating agents and lenalidomide. Selected patients respond to antithymocyte globulins, and thrombopoietin receptor agonists are under investigation in lower-risk MDS with thrombocytopenia. Some patients, while remaining at a “lower risk” MDS level, have severe cytopenias and/or poor prognostic factors, found using newer prognostic parameters, or resistance to treatment, making them urgent candidates for more intensive approaches, including allogeneic stem cell transplantation.

Myelodysplastic syndromes (MDSs) are clonal stem cell disorders characterized by ineffective hematopoiesis leading to blood cytopenias, and by a high incidence of progression to acute myeloid leukemia (AML).1  The pathophysiology of MDS is a multistep process involving genetic changes detectable by conventional cytogenetic techniques or smaller anomalies detectable only by more sophisticated methods like single nucleotide polymorphism array technology2-4  or sequencing techniques. Somatic mutations, now detected in most MDS cases,5  can involve genes encoding signaling molecules (NRAS, KRAS, CBL, JAK2, FLT3),5,6  epigenetic regulators (TET2, ASXL1, EZH2, UTX, IDH1, IDH2, DNMT3A, SETBP1),5,7-12  splicing factors (SF3B1, SRSF2, ZRSF2, U2AF1),13-16  and transcription regulators (RUNX1, NPM1 and TP53).5,17-19  Widespread gene hypermethylation, on the other hand, is a major finding during progression of MDS.20,21 

Main prognostic factors in MDS include the number and importance of cytopenias, marrow blasts percentage, and marrow cytogenetic abnormalities combined in a “classic” International Prognostic Scoring System (IPSS) (that was very recently revised [IPSS-R]) that distinguishes between various subgroups with significantly different risk of progression to AML and survival.22,23  Other prognostic factors include the presence of grade ≥2 marrow fibrosis,24  certain somatic gene mutations,5  and possibly some flow cytometry parameters,25  but the last 2 tests are currently not routinely performed in most laboratories.

Although the division is schematic, it is customary since publication of the classic IPSS to separate MDS into “higher risk” (corresponding to IPSS high or intermediate-2) and “lower risk” (corresponding to IPSS low or intermediate-1).22  Higher-risk MDS carry a major risk of progression to AML and short survival, and treatment in those patients should aim, whenever possible, to modify the natural disease course. Treatments used in higher-risk MDSs therefore include allogeneic stem cell transplantation (SCT); the hypomethylating agents (HMAs), azacitidine (AZA),26  and decitabine27 ; and, although currently less often, chemotherapy (mainly intensive anthracycline-AraC combinations).28  In lower-risk MDS, the risk of AML progression is less and survival is longer, with approximately one-half of those elderly patients dying from a cause other than the consequences of MDS or AML.29  In those patients, the main priority is generally the treatment of cytopenias, mainly of anemia (usually the predominant cytopenia), and the improvement in quality of life. Still, some of those patients may be identified, either rapidly by their revised IPSS score23  or by other biological characteristics,30  or subsequently by their resistance to first-line treatment as having a poorer prognosis, and they may benefit from treatments generally applied to higher-risk MDS.

Anemia, the predominant cytopenia in most cases of lower-risk MDS, is in general the primary focus. It often requires repeated red blood cell (RBC) transfusions, leading to potential iron overload.31 

Treatment of anemia

RBC transfusions or drugs?

Chronic RBC transfusions could be considered an only treatment of anemia of lower-risk MDS, because very few drugs are approved in this situation and none has clearly demonstrated that it could improve survival. However, chronic RBC transfusion are associated not so much with risks of viral infection or of alloimmunization, which are now very low, but with chronic anemia (ie, average hemoglobin levels <10 g/dL), leading to increased morbidity, especially as a result of cardiac failure, falls, fatigue, and lower quality of life.31,32  Transfusions are also time consuming for the patient, induce their “dependence” toward the medical system, and require the use of hospital beds, and their cost (including patient transportation, serum testing, iron chelation, etc.) is important, although generally lower than that of erythropoiesis stimulating agents (ESAs). Although this remains disputed (discussed later), iron overload as a result of RBC transfusions may also be deleterious to various organs.31,33  Finally, we and others also recently found that in lower-risk MDS with anemia, receiving ESAs had no impact on progression to AML but was an independent favorable prognostic factor for survival, although it was unclear whether this was because of ESA treatment itself or to maintaining hemoglobin levels >10 g/L or avoiding iron overload.34-37 

What is our first-line treatment of anemia in lower-risk MDS?

Patients without del(5q): ESAs.

ESAs (ie, recombinant erythropoietin (EPO) or darbepoetin [DAR]), remain the first choice of treatment of anemia in most lower-risk MDS without del(5q). Major favorable prognostic factors for response to ESAs are low or no RBC transfusion requirement (<2 U per month) and baseline serum EPO level <500 U/L.38  Most lower-risk MDS are now considered for anemia treatment when no or limited RBC transfusions are required, and in our experience their EPO level was <200 UI in 62% of the cases.34  Weekly doses of 40 000 units of EPO-α (Procrit or Eprex), 30 000 units of EPO-β (Recormon), or 150 to 300 μg of DAR (Aranesp) yield approximately 60% of erythroid responses, according to IWG 2006 response criteria,39  when the baseline EPO level is low and transfusion requirement is limited or absent,34-36,40,41  and response rates appear to be somewhat higher using 60 000 vs 30 000 U per week of EPO, and 300 μg vs 150 μg per week of DAR. The efficacy of ESAs can be further improved by the addition of granulocyte colony-stimulating factor (G-CSF),38,42  although to a lesser extent when high doses of ESAs (60 000 U/week of EPO or 300 μg/week of DAR) are used. Contrary to previous findings, we did not find in 2 large patient series that RARS or RCMD (+/−RS) responded less favorably to an ESA alone than to RA.34,43  Finally, there are no data showing that one ESA could be superior to another.

Most responses to ESA occur within 8 weeks of treatment, although some patients respond after only 12 weeks. During initial treatment, close monitoring of hemoglobin level is required to avoid increases to >12 g/dL, which is associated with a potential risk of systemic hypertension and thrombosis when ESAs are used to treat renal failure (although they have not been documented in MDS). Supplemental oral or intravenous iron is advocated mainly in case of relapse of anemia after initial response to ESAs, because prolonged ESA may lead to iron deficiency.44 

Median duration of response to ESA is approximately 2 years, and responses are longer in patients with major response according to IWG 2000 criteria,39  IPSS low or intermediate-1, marrow blasts <5%, and no multilineage dysplasia.34,35,45  Interestingly, approximately 70% of the relapses of anemia after initial response to ESAs are not associated with progression to higher-risk MDS but simply to loss of sensitivity of erythroid progenitors to ESAs,34  and second-line treatments in those patients may be different from those required in patients showing concomitant progression to higher-risk MDS.

Lower-risk MDS with del 5q: lenalidomide.

Anemia of lower-risk MDS with del 5q compared with that of other lower-risk MDS, showed lower response rates to ESA (39% vs 52% in our experience) and significantly shorter responses to ESA (median 1 year vs 2 years).46  However, it dramatically responds to lenalidomide (LEN), approved by Food and Drug Administration in this indication if anemia is transfusion-dependent (TD), based on 2 large studies (MDS 003 and 004 trials).47,48  In those trials, LEN (5-10 mg/day) yielded RBC transfusion-independence (RBC-TI) in 55% to 65% of the subjects, with a median duration of RBC-TI of 2 to 2.5 years.47,48  Cytogenetic response was achieved in 50% to 73% of subjects (including 30%-45% complete responses). Combining results of those 2 studies showed higher RBC-TI and cytogenetic responses with a daily dose of 10 mg (compared with 5 mg), less RBC-TI in patients with cytogenetic abnormalities in addition to del 5q, and high RBC-TD.49  TP53 gene mutations, found in about 20% of lower-risk MDS with del 5q, seem to confer resistance to LEN and a higher risk of AML progression,50  and their presence may require more aggressive treatment.

Grade 3 or 4 neutropenia and thrombocytopenia, seen in approximately 60% of the patients during the first weeks of treatment, constitute the most common adverse events of LEN.47,48  Close monitoring of blood counts is therefore required during this period, as well as drug discontinuation made if absolute neutrophil count decreases to <0.5G/L or platelets <25 G/L, and the drug restarted at a half dose upon correction of cytopenias.51  Addition of G-CSF can however be recommended if ANC <1 G/L, to avoid neutropenia and further dose reductions of LEN, because higher LEN doses may be associated with better erythroid and cytogenetic responses, as mentioned before.51  The thrombopoietin (TPO) receptor agonist romiplostim may reduce thrombocytopenia in that context, but it is not available for routine practice.52 

Other side effects of LEN in low-risk MDS with del 5q include deep venous thrombosis (DVT) and/or pulmonary embolism. Although DVT was observed in 8 of the 95 patients treated in the French patient–named program,53  it was reported in only 0.53% of more than 7500 MDS treated with LEN in a US postmarketing experience, but the incidence was higher in patients with concurrent use of EPO.54  Prophylactic measures for DVT in MDS treated with LEN are not codified, except in patients with a history of DVT, where prophylactic anticoagulation is probably justified.51 

Rash is frequent, although generally transient, with LEN in MDS, whereas diarrhea can be long lasting, with limited efficacy of symptomatic treatment.47,55 

In responders, the optimal duration of LEN treatment is unknown. Because stem cell with del(5q) persist in responders,56  prolonged treatment may be required to avoid rapid relapse. On the other hand, drug discontinuation in patients who have achieved complete cytogenetic response may be associated with prolonged responses.57 

Although Food and Drug Administration approved LEN in lower-risk MDS with del 5q, the European Medicines Agency raised concern over a potential risk of LEN to trigger AML progression in some lower-risk MDS with del 5q and requested additional analyses. In the absence of prospective randomized trials, 3 retrospective analyses comparing the long-term outcome of lower-risk MDS with del 5q treated with and without LEN found no excess risk of AML with lenalidomide.58,59  Until a new European Medicines Agency examination, therefore, EU investigators should use LEN as a second-line treatment, after ESA failure, and preferably in a clinical trial. Because LEN is currently approved only in the case of RBC-TD, patients with non-TD anemia may also be candidates for ESA. Furthermore, LEN is currently indicated only in cases of RBC TD anemia, and patients with lower-risk MDS with del 5q with non–TD anemia are also candidates for first-line treatment with ESAs.

What are our second-line treatments for anemia of lower-risk MDS?

Patients without del 5q.

Treatment after ESA failure (primary resistance or relapse after a response) in patients who remain with IPSS low or intermediate-1 score for MDS remains disappointing overall. Most patients eventually require long-term RBC transfusions. In our experience, early ESA failure (no response to ESA or relapse within 6 months) is a marker of disease severity associated with frequent subsequent AML progression and a median survival of only 3 years.60  In such patients, second-line treatments with a potential impact on disease course may need to be considered. The second-line treatments we currently use include antithymocyte globulin (ATG), HMAs, and LEN.

ATG: ATG, with or without cyclosporine, can yield an erythroid response (associated with response on other cytopenias, especially thrombocytopenia), in 25% to 40% of patients treated.61-66  Response rates, however, depend largely on the population treated. ATG results are better in relatively young (<65 years) low-risk MDS patients with a RBC transfusion history of <2 years, with normal karyotype (or possibly trisomy 8), with no excess blasts, HLA DR15 genotype, and possibly in patients with thrombocytopenia in addition to anemia, a small PNH clone, or marrow hypocellularity.61  In the French registry of MDS, the patients represented only 6.5% of the low-risk MDS, suggesting that good ATG candidates may be relatively rare in MDS.67  ATG may also be considered in patients in whom thrombocytopenia is the predominant cytopenia.

More recently, alemtuzumab treatment in 32 lower-risk MDS patients who had favorable criteria for ATG response, yielded a 77% response rate, sustained improvement in blood counts, and cytogenetic remissions.68  However, the drug is not widely available in this indication. In addition, this patient series was highly selected, because it included patients with good prognostic factors of response to immunosuppression.

Hypomethylating agents: HMAs have been reported to yield RBC-TI in 30% to 40% of patients69,70  and may also be effective for other cytopenias in lower-risk MDS. They are approved in this situation in several countries, including the United States. In a phase II trial randomizing AZA and AZA + EPO in RBC TD lower-risk MDS clearly identified as resistant to ESA; however, we observed only 17% RBC-TI, without difference between the 2 treatment arms, possibly suggesting lower efficacy in patients who are clearly ESA resistant.71 

We use HMAs as second-line treatment particularly in patients with thrombocytopenia in addition to anemia (or isolated thrombocytopenia). Even though they have not demonstrated a survival advantage in low-risk MDS, we also use them in patients with early ESA failure (no response or relapse within 6 months of response), whose progression rate and survival is rather unfavorable.60 

Lenalidomide: Lenalidomide yields RBC-TI in 25% to 30% of lower-risk MDS without del 5q resistant to ESA.72,73  Because it induces neutropenia and thrombocytopenia (although to a lesser extent than in patients with del 5q), it is difficult to use when those cytopenias are present in addition to anemia. Furthermore, it is unclear whether LEN, in addition to improving anemia, has any effect on disease progression and survival in those patients. Therefore, LEN, in non–del 5q patients, appears justified only in clinical trials. Preliminary results suggest that the combination of LEN and ESA may yield high RBC-TI rates in patients resistant to an ESA alone, and we are currently trying to confirm those results in a prospective, randomized trial.74 

Patients with del 5q: Results of the MDS 003 and MDS 004 trials47,48  (described before) also suggest that resistance to LEN in lower-risk MDS with del 5q is associated with poor prognosis, even if no immediate progression to high-risk MDS is observed. Patients with TP53 gene mutation may have a particularly poor outcome.50  Although no prospective data exist, those patients should probably be candidates to approaches having demonstrated a survival benefit in MDS, including HMAs, and whenever possible allogenic SCT. Our recent experience with AZA after LEN treatment failure showed a 50% response rate and a median overall survival of 32 months in responding patients.75 

Long term RBC transfusions and iron chelation therapy.

In many patients with lower-risk MDS, anemia will eventually become resistant to all available drug treatments, even in the absence of evolution to higher-risk MDS, and will require repeated RBC cell transfusions.60  For those patients, it is recommended to administer transfusions at sufficiently high hemoglobin thresholds (ie, at least 8 g/dL and 9 or 10 g/dL in cases of comorbidities worsened by anemia [eg, coronary artery disease, heart failure] or in cases of poor functional tolerance). In addition, a sufficient number of RBC concentrates should be transfused each time, over 2 or 3 days if needed, to increase the hemoglobin level to >10 g/dL and thereby limit the effects of chronic anemia.

A large debate exists about the deleterious effect of iron overload in MDS patients and whether iron chelation may be useful in patients with iron overload. In particular, although heart iron overload is a well-documented cause of heart failure in children with thalassemia,76,77  its incidence and clinical consequences are uncertain in RBC-transfused MDS patients, especially as many of them already have other causes of cardiac morbidity. Some authors may therefore consider that in those patients, iron overload is just one cause of cardiac failure,78  whereas others may consider that this additional cause may precipitate a sometimes unstable cardiac situation.79,80  Discrepancies are also probably related to the variable median number of RBC units transfused in different published series. Indeed, significant iron overload appears to occur later in the heart than in other organs, especially the liver. However, heart magnetic resonance imaging studies show that heart iron overload (reflected by a decrease in heart T2* imaging) is frequent in patients having received at least 70 to 80 RBC concentrates or more, a frequent situation in low-risk MDS, and that a heart T2* value <20 ms is associated with decreased left ventricular ejection fraction and a risk of heart failure.81 

Thus, very heavily RBC-transfused MDS patients develop major iron overload, especially in the heart and liver, which may reduce survival owing to cardiac failure or liver cirrhosis. It has been suggested in 3 retrospective studies that adequate chelation in highly transfused patients may improve their survival.82-84  Prospective, randomized studies are underway to confirm those results, but they are difficult to conduct because 2 chelating agents (deferoxamine and deferasirox) are approved for this indication in MDS.

In the absence of prospective studies, published recommendations for iron chelation therapy only result from expert opinions.85,86  We generally advocate starting chelation in patients with relatively favorable prognosis (ie, low or intermediate-1–risk MDS) who have received at least 50 to 60 RBC concentrates, or if serum ferritin increases >2500 U/L or if cardiac T2* findings are significantly reduced. Future candidates to allogeneic SCT are an exception. Indeed, although the underlying mechanisms are unclear, there is a consensus that even relatively moderate iron overload before allogeneic SCT is associated with increased transplant-related mortality.87-90  In addition, intensive chelation treatment before transplant may improve survival in those patients, although this was observed in a retrospective study only.91  Thus, in MDS patients who may eventually be candidates for allogeneic SCT, we start iron chelation after around 20 RBC concentrates or above a serum ferritin level of 1000 UI. The same thresholds have been advocated in low-risk MDS as a whole in some recommendations,85,86  but, as stated before, they not based on prospective studies.

Iron chelation is now made easier by the availability of oral iron chelators (especially deferasirox), in addition to the classical parenteral deferoxamine. However, deferasirox is frequently associated with gastrointestinal side effects and cannot be used in patients with renal failure.92  Deferiprone, another oral iron chelator, is currently not approved for MDS in most countries and can cause neutropenia in a small percentage of patients, a side effect that is problematic in MDS.93 

Treatment of neutropenia and thrombocytopenia

In lower-risk MDS, neutropenia and thrombocytopenia are less frequent than anemia and are infrequently isolated or profound.

Neutropenia.

WBC are <1.5 mm3 in only 7% of lower-risk MDS,94  and neutropenia is rarely associated with life-threatening infection if no drugs that worsen neutropenia are used. G-CSF and granulocyte macrophage–CSF can improve neutropenia in 60% to 75% of those cases, but their prolonged use has not demonstrated an impact on survival, whereas a risk of stimulating progression to higher-risk MDS or AML has not been formally excluded. They may be used for transient periods, in patients who have severe sepsis, but this has never occurred in our clinical practice. Immediate use of broad-spectrum antibiotics should be recommended to neutropenic MDS patients in case of fever or other signs of infection. In the absence of a previous infection episode with resistant strains, we ask our neutropenic patients to take amoxicilline-clavulanic acid and ciprofloxacin immediately in case of fever and then contact their physicians.

Thrombocytopenia.

Platelets <50 000/mm3 are seen in approximately 30% of low-risk MDS,94  and severe bleeding is relatively rare unless drugs interfering with hemostasis are used. We sometimes use high-dose androgens, which can improve thrombocytopenia in approximately one-third of thrombocytopenic lower-risk MDS, but the response is generally transient.95,96  Growth factors nonspecific of the platelet lineage, including interleukin (IL)3, IL6, and IL11, have been used with some success, but they also produce side effects.95,97,98 

In exceptional cases, a peripheral mechanism of platelet destruction may predominate in MDS, as evidenced by platelet lifespan studies, with a possible success of splenectomy in our experience.99 

Because TPO itself is immunogenic, leading to thrombocytopenia, TPO receptor agonists including romiplostim and eltrombopag have been designed to treat thrombocytopenias of different origins. Romiplostim at a high dose (500-1500 μg/week) yielded 55% platelet responses in a phase II trial in lower-risk MDS with thrombocytopenia.100  However, in approximately 15% of the patients, a transient rise in marrow blasts was seen but was reversible after drug discontinuation. In a randomized phase II study vs placebo in lower-risk MDS with thrombocytopenia, romiplostim reduced the incidence of severe bleeding and platelet transfusions, but there was a suspected increase in the risk of AML, and data are currently under review.101  Currently, TPO agonist receptors are unavailable for routine practice.

ATG and HMAs appear to elicit platelet response in 35% to 40% of the cases of lower-risk MDS, in addition to erythroid responses, and we sometimes use them in this context.62,66,102-104 

Although management of cytopenias, mainly anemia, is generally the major clinical objective in lower-risk MDS, some patients may have, at diagnosis or during evolution, features associated with a risk of progression to high-risk MDS/AML or life-threatening cytopenias that may justify treatment strategies aimed at modifying the disease course, especially with HMAs and, in some younger patients, even lead to the consideration of allogeneic SCT.

Identifying lower-risk MDS patients with poorer outcome

At presentation.

The “classical” IPSS,22  defining lower-risk MDS as low and intermediate-1 risk IPSS, appears to be insufficient, especially because it does not incorporate marrow multilineage dysplasia80  or RBC transfusion dependence,80  associated with poorer prognosis, as well as severity of thrombocytopenia,23  and because some cytogenetic abnormalities like those involving 3q21-26105  are considered an intermediate prognosis.

Some of those caveats are addressed by the World Health Organization classification–based Prognostic Scoring System,80  and more importantly by the revised IPSS,23  that appear to better discriminate prognosis in classical IPSS low and intermediate-1 MDS. For example, in IPSS low and intermediate-1 patients, 27% were shifted to higher-risk IPSS-R categories, mainly intermediate.23 

Presence of grade 2 or higher myelofibrosis in lower-risk MDS is also associated in some series with a higher risk of AML progression and poorer survival,24  although this parameter lacked prognostic significance in the IPSS-R cohort.23 

A M. D. Anderson scoring system for IPSS low and intermediate-1 patients, based on specific thresholds for platelets, hemoglobin, age, marrow blasts, and cytogenetics, was also capable of better discriminating the prognosis of IPSS lower-risk MDS,94  whereas presence of somatic mutations, especially of EZH2 gene, added independent poor prognostic value to this score.106 

During follow-up.

Lower-risk MDS patients (according to IPSS) who remain at lower risk but have early resistance to ESAs (non–del 5q patients)60  or resistance to LEN (del 5q patients),48  and who develop a cytogenetic abnormality or a life-threatening cytopenia (mainly thrombocytopenia) also have relatively poor survival.

How we manage those patients

Although prospective studies are lacking, we increasingly use HMAs (based on their known effect on survival in high-risk MDS) treatment in classic IPSS “lower-risk” patients.

We also consider allogeneic SCT in patients aged <60 to 65 years with a human leukocyte antigen (HLA)–identical donor and no contraindication to the procedure, in case of life-threatening thrombocytopenia; karyotype considered as unfavorable by IPSS-R (including 3q26 rearrangements); TP53, EZH2, or ASXL1 mutation; and in the absence of major response to HMAs (or subsequent relapse).

Chronic anemia remains the most frequent clinical problem in lower-risk MDS, which alters quality of life in those elderly patients. ESAs generally constitute the first-line treatment of anemia except in patients with 5q deletion, in whom results of LEN are superior, but responses to both treatments are generally transient. Second-line treatments of anemia (including HMAs, LEN in the absence of 5q deletion, and ATG) are less satisfactory, yielding at best one-third of responses, so that many patients eventually require repeated RBC transfusions, a situation in which indications for iron overload prophylaxis are still somewhat disputed. In a minority of lower-risk MDS, thrombocytopenia is the predominating cytopenia, and TPO agonist receptors are currently being tested in this situation, whereas HMAs or ATG may be useful. Some patients with lower-risk MDS according to IPSS may, at diagnosis or during evolution, have features associated with poorer prognosis, based on new prognostic scoring systems (eg, IPSS-R, M.D. Anderson score), presence of gene somatic mutations, or resistance to first-line treatment, that may consider them for more intensive treatment, including in some cases allogeneic SCT.

Contribution: P.F. and L.A. wrote the manuscript.

Conflict-of-interest disclosure: The authors declare no competing financial interests.

Correspondence: Pierre Fenaux, Groupe Francophone des Myelodysplasies, France, Hôpital Avicenne–Service d’Hématologie Clinique, Paris 13 university; or Assistance Publique, Hopitaux de Paris, 125 rue de Stalingrad, 93009 Bobigny, France; e-mail: [email protected].

1
Tefferi
 
A
Vardiman
 
JW
Myelodysplastic syndromes.
N Engl J Med
2009
, vol. 
361
 
19
(pg. 
1872
-
1885
)
2
Afable
 
MG
Wlodarski
 
M
Makishima
 
H
et al. 
SNP array-based karyotyping: differences and similarities between aplastic anemia and hypocellular myelodysplastic syndromes.
Blood
2011
, vol. 
117
 
25
(pg. 
6876
-
6884
)
3
Dunbar
 
AJ
Gondek
 
LP
O’Keefe
 
CL
et al. 
250K single nucleotide polymorphism array karyotyping identifies acquired uniparental disomy and homozygous mutations, including novel missense substitutions of c-Cbl, in myeloid malignancies.
Cancer Res
2008
, vol. 
68
 
24
(pg. 
10349
-
10357
)
4
Gondek
 
LP
Dunbar
 
AJ
Szpurka
 
H
McDevitt
 
MA
Maciejewski
 
JP
SNP array karyotyping allows for the detection of uniparental disomy and cryptic chromosomal abnormalities in MDS/MPD-U and MPD.
PLoS ONE
2007
, vol. 
2
 
11
pg. 
e1225
 
5
Bejar
 
R
Stevenson
 
K
Abdel-Wahab
 
O
et al. 
Clinical effect of point mutations in myelodysplastic syndromes.
N Engl J Med
2011
, vol. 
364
 
26
(pg. 
2496
-
2506
)
6
Paquette
 
RL
Landaw
 
EM
Pierre
 
RV
et al. 
N-ras mutations are associated with poor prognosis and increased risk of leukemia in myelodysplastic syndrome.
Blood
1993
, vol. 
82
 
2
(pg. 
590
-
599
)
7
Delhommeau
 
F
Dupont
 
S
Della Valle
 
V
et al. 
Mutation in TET2 in myeloid cancers.
N Engl J Med
2009
, vol. 
360
 
22
(pg. 
2289
-
2301
)
8
Kosmider
 
O
Gelsi-Boyer
 
V
Cheok
 
M
et al. 
Groupe Francophone des Myélodysplasies
TET2 mutation is an independent favorable prognostic factor in myelodysplastic syndromes (MDSs).
Blood
2009
, vol. 
114
 
15
(pg. 
3285
-
3291
)
9
Gelsi-Boyer
 
V
Trouplin
 
V
Adélaïde
 
J
et al. 
Mutations of polycomb-associated gene ASXL1 in myelodysplastic syndromes and chronic myelomonocytic leukaemia.
Br J Haematol
2009
, vol. 
145
 
6
(pg. 
788
-
800
)
10
Kosmider
 
O
Gelsi-Boyer
 
V
Slama
 
L
et al. 
Mutations of IDH1 and IDH2 genes in early and accelerated phases of myelodysplastic syndromes and MDS/myeloproliferative neoplasms.
Leukemia
2010
, vol. 
24
 
5
(pg. 
1094
-
1096
)
11
Thol
 
F
Weissinger
 
EM
Krauter
 
J
et al. 
IDH1 mutations in patients with myelodysplastic syndromes are associated with an unfavorable prognosis.
Haematologica
2010
, vol. 
95
 
10
(pg. 
1668
-
1674
)
12
Damm
 
F
Itzykson
 
R
Kosmider
 
O
et al. 
SETBP1 mutations in 658 patients with myelodysplastic syndromes, chronic myelomonocytic leukemia and secondary acute myeloid leukemias.
Leukemia
2013
 
Feb 5. doi: 10.1038/leu.2013.35. [Epub ahead of print]
13
Yoshida
 
K
Sanada
 
M
Shiraishi
 
Y
et al. 
Frequent pathway mutations of splicing machinery in myelodysplasia.
Nature
2011
, vol. 
478
 
7367
(pg. 
64
-
69
)
14
Papaemmanuil
 
E
Cazzola
 
M
Boultwood
 
J
et al. 
Chronic Myeloid Disorders Working Group of the International Cancer Genome Consortium
Somatic SF3B1 mutation in myelodysplasia with ring sideroblasts.
N Engl J Med
2011
, vol. 
365
 
15
(pg. 
1384
-
1395
)
15
Malcovati
 
L
Papaemmanuil
 
E
Bowen
 
DT
et al. 
Chronic Myeloid Disorders Working Group of the International Cancer Genome Consortium and of the Associazione Italiana per la Ricerca sul Cancro Gruppo Italiano Malattie Mieloproliferative
Clinical significance of SF3B1 mutations in myelodysplastic syndromes and myelodysplastic/myeloproliferative neoplasms.
Blood
2011
, vol. 
118
 
24
(pg. 
6239
-
6246
)
16
Patnaik
 
MM
Lasho
 
TL
Hodnefield
 
JM
et al. 
SF3B1 mutations are prevalent in myelodysplastic syndromes with ring sideroblasts but do not hold independent prognostic value.
Blood
2012
, vol. 
119
 
2
(pg. 
569
-
572
)
17
Christiansen
 
DH
Andersen
 
MK
Pedersen-Bjergaard
 
J
Mutations of AML1 are common in therapy-related myelodysplasia following therapy with alkylating agents and are significantly associated with deletion or loss of chromosome arm 7q and with subsequent leukemic transformation.
Blood
2004
, vol. 
104
 
5
(pg. 
1474
-
1481
)
18
Harada
 
H
Harada
 
Y
Niimi
 
H
Kyo
 
T
Kimura
 
A
Inaba
 
T
High incidence of somatic mutations in the AML1/RUNX1 gene in myelodysplastic syndrome and low blast percentage myeloid leukemia with myelodysplasia.
Blood
2004
, vol. 
103
 
6
(pg. 
2316
-
2324
)
19
Kaneko
 
H
Misawa
 
S
Horiike
 
S
Nakai
 
H
Kashima
 
K
TP53 mutations emerge at early phase of myelodysplastic syndrome and are associated with complex chromosomal abnormalities.
Blood
1995
, vol. 
85
 
8
(pg. 
2189
-
2193
)
20
Shen
 
L
Kantarjian
 
H
Guo
 
Y
et al. 
DNA methylation predicts survival and response to therapy in patients with myelodysplastic syndromes.
J Clin Oncol
2010
, vol. 
28
 
4
(pg. 
605
-
613
)
21
Jiang
 
Y
Dunbar
 
A
Gondek
 
LP
et al. 
Aberrant DNA methylation is a dominant mechanism in MDS progression to AML.
Blood
2009
, vol. 
113
 
6
(pg. 
1315
-
1325
)
22
Greenberg
 
P
Cox
 
C
LeBeau
 
MM
et al. 
International scoring system for evaluating prognosis in myelodysplastic syndromes.
Blood
1997
, vol. 
89
 
6
(pg. 
2079
-
2088
)
23
Greenberg
 
PL
Tuechler
 
H
Schanz
 
J
et al. 
Revised International Prognostic Scoring System (IPSS-R) for myelodysplastic syndromes.
Blood
2012
, vol. 
120
 
12
(pg. 
2454
-
2465
)
24
Della Porta
 
MG
Malcovati
 
L
Boveri
 
E
et al. 
Clinical relevance of bone marrow fibrosis and CD34-positive cell clusters in primary myelodysplastic syndromes.
J Clin Oncol
2009
, vol. 
27
 
5
(pg. 
754
-
762
)
25
van de Loosdrecht
 
AA
Westers
 
TM
Westra
 
AH
Drager
 
AM
van der Velden
 
VH
Ossenkoppele
 
GJ
Identification of distinct prognostic subgroups in low and intermediate-1 risk myelodysplastic syndromes by flow cytometry.
Blood
2008
, vol. 
111
 
3
(pg. 
1067
-
1077
)
26
Fenaux
 
P
Mufti
 
GJ
Hellstrom-Lindberg
 
E
et al. 
International Vidaza High-Risk MDS Survival Study Group
Efficacy of azacitidine compared with that of conventional care regimens in the treatment of higher-risk myelodysplastic syndromes: a randomised, open-label, phase III study.
Lancet Oncol
2009
, vol. 
10
 
3
(pg. 
223
-
232
)
27
Lübbert
 
M
Suciu
 
S
Baila
 
L
et al. 
Low-dose decitabine versus best supportive care in elderly patients with intermediate- or high-risk myelodysplastic syndrome (MDS) ineligible for intensive chemotherapy: final results of the randomized phase III study of the European Organisation for Research and Treatment of Cancer Leukemia Group and the German MDS Study Group.
J Clin Oncol
2011
, vol. 
29
 
15
(pg. 
1987
-
1996
)
28
Estey
 
EH
Thall
 
PF
Cortes
 
JE
et al. 
Comparison of idarubicin + ara-C-, fludarabine + ara-C-, and topotecan + ara-C-based regimens in treatment of newly diagnosed acute myeloid leukemia, refractory anemia with excess blasts in transformation, or refractory anemia with excess blasts.
Blood
2001
, vol. 
98
 
13
(pg. 
3575
-
3583
)
29
Guardiola
 
P
Runde
 
V
Bacigalupo
 
A
et al. 
Subcommittee for Myelodysplastic Syndromes of the Chronic Leukaemia Working Group of the European Blood and Marrow Transplantation Group
Retrospective comparison of bone marrow and granulocyte colony-stimulating factor-mobilized peripheral blood progenitor cells for allogeneic stem cell transplantation using HLA identical sibling donors in myelodysplastic syndromes.
Blood
2002
, vol. 
99
 
12
(pg. 
4370
-
4378
)
30
Mittelman
 
M
Oster
 
HS
Hoffman
 
M
Neumann
 
D
The lower risk MDS patient at risk of rapid progression.
Leuk Res
2010
, vol. 
34
 
12
(pg. 
1551
-
1555
)
31
Fenaux
 
P
Rose
 
C
Impact of iron overload in myelodysplastic syndromes.
Blood Rev
2009
, vol. 
23
 
Suppl 1
(pg. 
S15
-
S19
)
32
Crawford
 
J
Cella
 
D
Cleeland
 
CS
et al. 
Relationship between changes in hemoglobin level and quality of life during chemotherapy in anemic cancer patients receiving epoetin alfa therapy.
Cancer
2002
, vol. 
95
 
4
(pg. 
888
-
895
)
33
Roy
 
NB
Myerson
 
S
Schuh
 
AH
et al. 
Cardiac iron overload in transfusion-dependent patients with myelodysplastic syndromes.
Br J Haematol
2011
, vol. 
154
 
4
(pg. 
521
-
524
)
34
Park
 
S
Grabar
 
S
Kelaidi
 
C
et al. 
GFM group (Groupe Francophone des Myélodysplasies)
Predictive factors of response and survival in myelodysplastic syndrome treated with erythropoietin and G-CSF: the GFM experience.
Blood
2008
, vol. 
111
 
2
(pg. 
574
-
582
)
35
Jädersten
 
M
Malcovati
 
L
Dybedal
 
I
et al. 
Erythropoietin and granulocyte-colony stimulating factor treatment associated with improved survival in myelodysplastic syndrome.
J Clin Oncol
2008
, vol. 
26
 
21
(pg. 
3607
-
3613
)
36
Greenberg
 
PL
Sun
 
Z
Miller
 
KB
et al. 
Treatment of myelodysplastic syndrome patients with erythropoietin with or without granulocyte colony-stimulating factor: results of a prospective randomized phase 3 trial by the Eastern Cooperative Oncology Group (E1996).
Blood
2009
, vol. 
114
 
12
(pg. 
2393
-
2400
)
37
Golshayan
 
AR
Jin
 
T
Maciejewski
 
J
et al. 
Efficacy of growth factors compared to other therapies for low-risk myelodysplastic syndromes.
Br J Haematol
2007
, vol. 
137
 
2
(pg. 
125
-
132
)
38
Hellström-Lindberg
 
E
Gulbrandsen
 
N
Lindberg
 
G
et al. 
Scandinavian MDS Group
A validated decision model for treating the anaemia of myelodysplastic syndromes with erythropoietin + granulocyte colony-stimulating factor: significant effects on quality of life.
Br J Haematol
2003
, vol. 
120
 
6
(pg. 
1037
-
1046
)
39
Cheson
 
BD
Greenberg
 
PL
Bennett
 
JM
et al. 
Clinical application and proposal for modification of the International Working Group (IWG) response criteria in myelodysplasia.
Blood
2006
, vol. 
108
 
2
(pg. 
419
-
425
)
40
Mannone
 
L
Gardin
 
C
Quarre
 
MC
et al. 
Groupe Francais des Myelodysplasies
High-dose darbepoetin alpha in the treatment of anaemia of lower risk myelodysplastic syndrome results of a phase II study.
Br J Haematol
2006
, vol. 
133
 
5
(pg. 
513
-
519
)
41
Gabrilove
 
J
Paquette
 
R
Lyons
 
RM
Mushtaq
 
C
Sekeres
 
MA
Tomita
 
D
Dreiling
 
L
Phase 2, single-arm trial to evaluate the effectiveness of darbepoetin alfa for correcting anaemia in patients with myelodysplastic syndromes.
Br J Haematol
2008
, vol. 
142
 
3
(pg. 
379
-
393
)
42
Hellström-Lindberg
 
E
Birgegård
 
G
Carlsson
 
M
et al. 
A combination of granulocyte colony-stimulating factor and erythropoietin may synergistically improve the anaemia in patients with myelodysplastic syndromes.
Leuk Lymphoma
1993
, vol. 
11
 
3-4
(pg. 
221
-
228
)
43
Kelaidi
 
C
Beyne-Rauzy
 
O
Braun
 
T
et al. 
High response rate and improved exercise capacity and quality of life with a new regimen of darbepoetin alfa with or without filgrastim in lower-risk myelodysplastic syndromes: a phase II study by the GFM.
Ann Hematol
2013
 
(in press)
44
Thomas
 
C
Thomas
 
L
Biochemical markers and hematologic indices in the diagnosis of functional iron deficiency.
Clin Chem
2002
, vol. 
48
 
7
(pg. 
1066
-
1076
)
45
Jädersten
 
M
Montgomery
 
SM
Dybedal
 
I
Porwit-MacDonald
 
A
Hellström-Lindberg
 
E
Long-term outcome of treatment of anemia in MDS with erythropoietin and G-CSF.
Blood
2005
, vol. 
106
 
3
(pg. 
803
-
811
)
46
Kelaidi
 
C
Park
 
S
Brechignac
 
S
et al. 
Groupe Francophone des Myélodysplasies (GFM)
Treatment of myelodysplastic syndromes with 5q deletion before the lenalidomide era; the GFM experience with EPO and thalidomide.
Leuk Res
2008
, vol. 
32
 
7
(pg. 
1049
-
1053
)
47
List
 
A
Dewald
 
G
Bennett
 
J
et al. 
Myelodysplastic Syndrome-003 Study Investigators
Lenalidomide in the myelodysplastic syndrome with chromosome 5q deletion.
N Engl J Med
2006
, vol. 
355
 
14
(pg. 
1456
-
1465
)
48
Fenaux
 
P
Giagounidis
 
A
Selleslag
 
D
et al. 
MDS-004 Lenalidomide del5q Study Group
A randomized phase 3 study of lenalidomide versus placebo in RBC transfusion-dependent patients with low-/intermediate-1-risk myelodysplastic syndromes with del5q.
Blood
2011
, vol. 
118
 
14
(pg. 
3765
-
3776
)
49
List
 
A
Giagounidis
 
A
Backstrom
 
J
Fu
 
T
Fenaux
 
P
Early lenalidomide (LEN) dose intensity and durable RBC-transfusion independence (RBC-TI) in patients (pts) with low-/int-1-risk myelodysplastic syndromes (MDS) and del5q.
J Clin Oncol
2011
, vol. 
29
 pg. 
6522
 
50
Jädersten
 
M
Saft
 
L
Smith
 
A
et al. 
TP53 mutations in low-risk myelodysplastic syndromes with del(5q) predict disease progression.
J Clin Oncol
2011
, vol. 
29
 
15
(pg. 
1971
-
1979
)
51
Giagounidis
 
A
Fenaux
 
P
Mufti
 
GJ
et al. 
Practical recommendations on the use of lenalidomide in the management of myelodysplastic syndromes.
Ann Hematol
2008
, vol. 
87
 
5
(pg. 
345
-
352
)
52
Wang
 
ES
Lyons
 
RM
Larson
 
RA
et al. 
A randomized, double-blind, placebo-controlled phase 2 study evaluating the efficacy and safety of romiplostim treatment of patients with low or intermediate-1 risk myelodysplastic syndrome receiving lenalidomide.
J Hematol Oncol
2012
, vol. 
5
 pg. 
71
 
53
Le Bras
 
F
Sebert
 
M
Kelaidi
 
C
et al. 
Treatment by lenalidomide in lower risk myelodysplastic syndrome with 5q deletion—the GFM experience.
Leuk Res
2011
, vol. 
35
 
11
(pg. 
1444
-
1448
)
54
Yang
 
X
Brandenburg
 
NA
Freeman
 
J
et al. 
Venous thromboembolism in myelodysplastic syndrome patients receiving lenalidomide: results from postmarketing surveillance and data mining techniques.
Clin Drug Investig
2009
, vol. 
29
 
3
(pg. 
161
-
171
)
55
List
 
A
Kurtin
 
S
Roe
 
DJ
et al. 
Efficacy of lenalidomide in myelodysplastic syndromes.
N Engl J Med
2005
, vol. 
352
 
6
(pg. 
549
-
557
)
56
Tehranchi
 
R
Woll
 
PS
Anderson
 
K
et al. 
Persistent malignant stem cells in del(5q) myelodysplasia in remission.
N Engl J Med
2010
, vol. 
363
 
11
(pg. 
1025
-
1037
)
57
Giagounidis
 
AA
Kulasekararaj
 
A
Germing
 
U
et al. 
Long-term transfusion independence in del(5q) MDS patients who discontinue lenalidomide.
Leukemia
2012
, vol. 
26
 
4
(pg. 
855
-
858
)
58
Adès
 
L
Le Bras
 
F
Sebert
 
M
et al. 
Treatment with lenalidomide does not appear to increase the risk of progression in lower risk myelodysplastic syndromes with 5q deletion. A comparative analysis by the Groupe Francophone des Myelodysplasies.
Haematologica
2012
, vol. 
97
 
2
(pg. 
213
-
218
)
59
Kuendgen
 
A
Lauseker
 
M
List
 
AF
et al. 
Lenalidomide does not increase AML progression risk in RBC transfusion-dependent patients with Low- or Intermediate-1-risk MDS with del(5q): a comparative analysis.
Leukemia
2012
 
Dec 21. doi: 10.1038/leu.2012.369. [Epub ahead of print]
60
Kelaidi
 
C
Park
 
S
Sapena
 
R
et al. 
Long-term outcome of anemic lower-risk myelodysplastic syndromes without 5q deletion refractory to or relapsing after erythropoiesis-stimulating agents.
Leukemia
2013
 
Jan 16. doi: 10.1038/leu.2013.16. [Epub ahead of print]
61
Saunthararajah
 
Y
Nakamura
 
R
Wesley
 
R
Wang
 
QJ
Barrett
 
AJ
A simple method to predict response to immunosuppressive therapy in patients with myelodysplastic syndrome.
Blood
2003
, vol. 
102
 
8
(pg. 
3025
-
3027
)
62
Yazji
 
S
Giles
 
FJ
Tsimberidou
 
AM
et al. 
Antithymocyte globulin (ATG)-based therapy in patients with myelodysplastic syndromes.
Leukemia
2003
, vol. 
17
 
11
(pg. 
2101
-
2106
)
63
Passweg
 
JR
Giagounidis
 
AA
Simcock
 
M
et al. 
Immunosuppressive therapy for patients with myelodysplastic syndrome: a prospective randomized multicenter phase III trial comparing antithymocyte globulin plus cyclosporine with best supportive care—SAKK 33/99.
J Clin Oncol
2011
, vol. 
29
 
3
(pg. 
303
-
309
)
64
Molldrem
 
JJ
Caples
 
M
Mavroudis
 
D
Plante
 
M
Young
 
NS
Barrett
 
AJ
Antithymocyte globulin for patients with myelodysplastic syndrome.
Br J Haematol
1997
, vol. 
99
 
3
(pg. 
699
-
705
)
65
Sloand
 
EM
Wu
 
CO
Greenberg
 
P
Young
 
N
Barrett
 
J
Factors affecting response and survival in patients with myelodysplasia treated with immunosuppressive therapy.
J Clin Oncol
2008
, vol. 
26
 
15
(pg. 
2505
-
2511
)
66
Lim
 
ZY
Killick
 
S
Germing
 
U
et al. 
Low IPSS score and bone marrow hypocellularity in MDS patients predict hematological responses to antithymocyte globulin.
Leukemia
2007
, vol. 
21
 
7
(pg. 
1436
-
1441
)
67
Cereja
 
S
Brechignac
 
S
Ades
 
L
et al. 
Should immunosuppressive therapy (IST) be used more often in lower risk MDS?
ASH Annual Meeting Abstracts
2010
, vol. 
116
 
21
pg. 
1868
 
68
Sloand
 
EM
Olnes
 
MJ
Shenoy
 
A
et al. 
Alemtuzumab treatment of intermediate-1 myelodysplasia patients is associated with sustained improvement in blood counts and cytogenetic remissions.
J Clin Oncol
2010
, vol. 
28
 
35
(pg. 
5166
-
5173
)
69
Silverman
 
LR
McKenzie
 
DR
Peterson
 
BL
et al. 
Cancer and Leukemia Group B
Further analysis of trials with azacitidine in patients with myelodysplastic syndrome: studies 8421, 8921, and 9221 by the Cancer and Leukemia Group B.
J Clin Oncol
2006
, vol. 
24
 
24
(pg. 
3895
-
3903
)
70
Silverman
 
LR
Demakos
 
EP
Peterson
 
BL
et al. 
Randomized controlled trial of azacitidine in patients with the myelodysplastic syndrome: a study of the cancer and leukemia group B.
J Clin Oncol
2002
, vol. 
20
 
10
(pg. 
2429
-
2440
)
71
Boehrer
 
S
Beyne-Rauzy
 
O
Prebet
 
T
et al. 
Interim results of a randomized phase II trial of azacitidine (AZA) +/− Epo in lower risk myelodysplastic syndrome (MDS) resistant to an erythropoietic stimulating agent (ESA) alone.
Blood
2010
, vol. 
116
 
21
(pg. 
784
-
784
)
72
Raza
 
A
Reeves
 
JA
Feldman
 
EJ
et al. 
Phase 2 study of lenalidomide in transfusion-dependent, low-risk, and intermediate-1 risk myelodysplastic syndromes with karyotypes other than deletion 5q.
Blood
2008
, vol. 
111
 
1
(pg. 
86
-
93
)
73
Sibon
 
D
Cannas
 
G
Baracco
 
F
et al. 
Groupe Francophone des Myélodysplasies
Lenalidomide in lower-risk myelodysplastic syndromes with karyotypes other than deletion 5q and refractory to erythropoiesis-stimulating agents.
Br J Haematol
2012
, vol. 
156
 
5
(pg. 
619
-
625
)
74
Komrokji
 
RS
Lancet
 
JE
Swern
 
AS
et al. 
Combined treatment with lenalidomide and epoetin alfa in lower-risk patients with myelodysplastic syndrome.
Blood
2012
, vol. 
120
 
17
(pg. 
3419
-
3424
)
75
Komrokji
 
RS
Bally
 
C
Itzykson
 
R
et al. 
Azacitidine treatment for lenalidomide (LEN)-resistant myelodysplastic syndrome (MDS) with del 5q.
ASH Annual Meeting Abstracts
2012
, vol. 
120
 
21
pg. 
3833
 
76
Davis
 
BA
O’Sullivan
 
C
Jarritt
 
PH
Porter
 
JB
Value of sequential monitoring of left ventricular ejection fraction in the management of thalassemia major.
Blood
2004
, vol. 
104
 
1
(pg. 
263
-
269
)
77
Kirk
 
P
Roughton
 
M
Porter
 
JB
et al. 
Cardiac T2* magnetic resonance for prediction of cardiac complications in thalassemia major.
Circulation
2009
, vol. 
120
 
20
(pg. 
1961
-
1968
)
78
Chee
 
CE
Steensma
 
DP
Wu
 
W
Hanson
 
CA
Tefferi
 
A
Neither serum ferritin nor the number of red blood cell transfusions affect overall survival in refractory anemia with ringed sideroblasts.
Am J Hematol
2008
, vol. 
83
 
8
(pg. 
611
-
613
)
79
Malcovati
 
L
Porta
 
MG
Pascutto
 
C
et al. 
Prognostic factors and life expectancy in myelodysplastic syndromes classified according to WHO criteria: a basis for clinical decision making.
J Clin Oncol
2005
, vol. 
23
 
30
(pg. 
7594
-
7603
)
80
Malcovati
 
L
Germing
 
U
Kuendgen
 
A
et al. 
Time-dependent prognostic scoring system for predicting survival and leukemic evolution in myelodysplastic syndromes.
J Clin Oncol
2007
, vol. 
25
 
23
(pg. 
3503
-
3510
)
81
Jensen
 
PD
Jensen
 
FT
Christensen
 
T
Eiskjaer
 
H
Baandrup
 
U
Nielsen
 
JL
Evaluation of myocardial iron by magnetic resonance imaging during iron chelation therapy with deferrioxamine: indication of close relation between myocardial iron content and chelatable iron pool.
Blood
2003
, vol. 
101
 
11
(pg. 
4632
-
4639
)
82
Rose
 
C
Brechignac
 
S
Vassilief
 
D
et al. 
GFM (Groupe Francophone des Myélodysplasies)
Does iron chelation therapy improve survival in regularly transfused lower risk MDS patients? A multicenter study by the GFM (Groupe Francophone des Myélodysplasies).
Leuk Res
2010
, vol. 
34
 
7
(pg. 
864
-
870
)
83
Leitch
 
HA
Improving clinical outcome in patients with myelodysplastic syndrome and iron overload using iron chelation therapy.
Leuk Res
2007
, vol. 
31
 
Suppl 3
(pg. 
S7
-
S9
)
84
Neukirchen
 
J
Fox
 
F
Kündgen
 
A
et al. 
Improved survival in MDS patients receiving iron chelation therapy—a matched pair analysis of 188 patients from the Düsseldorf MDS registry.
Leuk Res
2012
, vol. 
36
 
8
(pg. 
1067
-
1070
)
85
Gattermann
 
N
Porter
 
J
Lopes
 
L
Seymour
 
J
Consensus statement on iron overload in myelodysplastic syndromes.
Hematol Oncol Clin North Am
2005
, vol. 
19
 
suppl 1
(pg. 
18
-
25
)
86
Greenberg
 
PL
Myelodysplastic syndromes: iron overload consequences and current chelating therapies.
J Natl Compr Canc Netw
2006
, vol. 
4
 
1
(pg. 
91
-
96
)
87
Alessandrino
 
EP
Della Porta
 
MG
Bacigalupo
 
A
et al. 
Prognostic impact of pre-transplantation transfusion history and secondary iron overload in patients with myelodysplastic syndrome undergoing allogeneic stem cell transplantation: a study from the Gruppo Italiano Trapianto di Midollo Osseo (GITMO).
Haematologica
2019
, vol. 
95
 
3
(pg. 
476
-
484
)
88
Armand
 
P
Kim
 
HT
Cutler
 
CS
et al. 
Prognostic impact of elevated pretransplantation serum ferritin in patients undergoing myeloablative stem cell transplantation.
Blood
2007
, vol. 
109
 
10
(pg. 
4586
-
4588
)
89
Pullarkat
 
V
Objectives of iron chelation therapy in myelodysplastic syndromes: more than meets the eye?
Blood
2009
, vol. 
114
 
26
(pg. 
5251
-
5255
)
90
Platzbecker
 
U
Bornhäuser
 
M
Germing
 
U
et al. 
Red blood cell transfusion dependence and outcome after allogeneic peripheral blood stem cell transplantation in patients with de novo myelodysplastic syndrome (MDS).
Biol Blood Marrow Transplant
2008
, vol. 
14
 
11
(pg. 
1217
-
1225
)
91
Lee
 
JW
Kang
 
HJ
Kim
 
EK
Kim
 
H
Shin
 
HY
Ahn
 
HS
Effect of iron overload and iron-chelating therapy on allogeneic hematopoietic SCT in children.
Bone Marrow Transplant
2009
, vol. 
44
 
12
(pg. 
793
-
797
)
92
Gattermann
 
N
Finelli
 
C
Porta
 
MD
et al. 
EPIC study investigators
Deferasirox in iron-overloaded patients with transfusion-dependent myelodysplastic syndromes: results from the large 1-year EPIC study.
Leuk Res
2010
, vol. 
34
 
9
(pg. 
1143
-
1150
)
93
Cermak
 
J
Jonasova
 
A
Vondrakova
 
J
et al. 
Efficacy and safety of administration of oral iron chelator deferiprone in patients with early myelodysplastic syndrome.
Hemoglobin
2011
, vol. 
35
 
3
(pg. 
217
-
227
)
94
Garcia-Manero
 
G
Shan
 
J
Faderl
 
S
et al. 
A prognostic score for patients with lower risk myelodysplastic syndrome.
Leukemia
2008
, vol. 
22
 
3
(pg. 
538
-
543
)
95
Montero
 
AJ
Estrov
 
Z
Freireich
 
EJ
Khouri
 
IF
Koller
 
CA
Kurzrock
 
R
Phase II study of low-dose interleukin-11 in patients with myelodysplastic syndrome.
Leuk Lymphoma
2006
, vol. 
47
 
10
(pg. 
2049
-
2054
)
96
Wattel
 
E
Cambier
 
N
Caulier
 
MT
Sautière
 
D
Bauters
 
F
Fenaux
 
P
Androgen therapy in myelodysplastic syndromes with thrombocytopenia: a report on 20 cases.
Br J Haematol
1994
, vol. 
87
 
1
(pg. 
205
-
208
)
97
Wu
 
HH
Talpaz
 
M
Champlin
 
RE
Pilat
 
SR
Kurzrock
 
R
Sequential interleukin 3 and granulocyte-macrophage-colony stimulating factor therapy in patients with bone marrow failure with long-term follow-up of responses.
Cancer
2003
, vol. 
98
 
11
(pg. 
2410
-
2419
)
98
Gordon
 
MS
Nemunaitis
 
J
Hoffman
 
R
et al. 
A phase I trial of recombinant human interleukin-6 in patients with myelodysplastic syndromes and thrombocytopenia.
Blood
1995
, vol. 
85
 
11
(pg. 
3066
-
3076
)
99
Bourgeois
 
E
Caulier
 
MT
Rose
 
C
Dupriez
 
B
Bauters
 
F
Fenaux
 
P
Role of splenectomy in the treatment of myelodysplastic syndromes with peripheral thrombocytopenia: a report on six cases.
Leukemia
2001
, vol. 
15
 
6
(pg. 
950
-
953
)
100
Kantarjian
 
H
Fenaux
 
P
Sekeres
 
MA
et al. 
Safety and efficacy of romiplostim in patients with lower-risk myelodysplastic syndrome and thrombocytopenia.
J Clin Oncol
2010
, vol. 
28
 
3
(pg. 
437
-
444
)
101
Kantarjian
 
HM
Mufti
 
GJ
Fenaux
 
P
et al. 
Treatment with the thrombopoietin (TPO)-receptor agonist romiplostim in thrombocytopenic patients (Pts) with low or intermediate-1 (int-1) risk myelodysplastic syndrome (MDS): follow-up AML and survival results of a randomized, double-blind, placebo (PBO)-controlled study.
ASH Annual Meeting Abstracts
2012
, vol. 
120
 
21
pg. 
421
 
102
Broliden
 
PA
Dahl
 
IM
Hast
 
R
et al. 
Antithymocyte globulin and cyclosporine A as combination therapy for low-risk non-sideroblastic myelodysplastic syndromes.
Haematologica
2006
, vol. 
91
 
5
(pg. 
667
-
670
)
103
Stadler
 
M
Germing
 
U
Kliche
 
KO
et al. 
A prospective, randomised, phase II study of horse antithymocyte globulin vs rabbit antithymocyte globulin as immune-modulating therapy in patients with low-risk myelodysplastic syndromes.
Leukemia
2004
, vol. 
18
 
3
(pg. 
460
-
465
)
104
Passweg
 
JR
Giagounidis
 
A
Simcock
 
M
et al. 
Immunosuppression for patients with low and intermediate risk myelodysplastic syndrome: a prospective randomized multicenter trial comparing antithymocyte globulin + cyclosporine with best supportive care: SAKK 33/99.
ASH Annual Meeting Abstracts
2007
, vol. 
110
 
11
pg. 
1461
 
105
Haase
 
D
Germing
 
U
Schanz
 
J
et al. 
New insights into the prognostic impact of the karyotype in MDS and correlation with subtypes: evidence from a core dataset of 2124 patients.
Blood
2007
, vol. 
110
 
13
(pg. 
4385
-
4395
)
106
Bejar
 
R
Stevenson
 
KE
Caughey
 
BA
et al. 
Validation of a prognostic model and the impact of mutations in patients with lower-risk myelodysplastic syndromes.
J Clin Oncol
2012
, vol. 
30
 
27
(pg. 
3376
-
3382
)
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