Abstract

Abstract 5186

Introduction:

Beta Thalassemia (β-thalassemia) is one of the more common hemoglobinopathies worldwide, being the heterozygous variant, called Beta Thalassemia Trait, a benign variant, but important to diagnose, for genetic counseling, trying to avoid the homozygous variant, called major.

Diagnostic of Beta Thalassemia Trait:

Classic testing for β-thalassemia includes: hematologic testing of red blood cell indices, peripheral blood smear (prewsence of target cells and RBC with basophilic stippling, etc.), and qualitative and quantitative hemoglobin analysis. Have been proposed too Discriminant functions, like the one published many years ago, by England and Fraser.

Objective:

Recently have been developed new parameters and information in the new automated hematology analyzer called DxH8008™ from Beckman Coulter as @MSCV, @RSF, @MAF, @ LHD% and many morphological parameters for RBC and Reticulocytes calles Cell Population Data. All this parameters may be used to create flagging for laboratory use only (LUO) or Research use only (RUO). The purpose of this study is to investigate the possible use or utility of this new information for the screening/flagging of Beta Thalassemia Trait.

Patient and Methods:

We have collected 30 patients with Beta Thalassemia Trait. All of them were confirmed by red cell morphology, Hgb Electroforesis, cromatography in liquid phase in human whole blood for the determination of Hemoglobin A2, F, A1c, and identification of abnormal hemoglobins and DNA analysis (DNA Analysis by GAP-PCR). We have compared these patients with a control group (184 individuals) and with other anemias (see Table 1).

Table 1
Diagnosticn
NON BETA (control) 184 
HbH (alfa thalasemia intermedia) 129 
Alfa Thalassemia Heterozygous 48 
Beta Thalassemia Trait (study group) 30 
Hereditary Spherocytosis 28 
Iron Deficiency Anaemia 22 
Thalassemia intermedia (Beta-Thal Interm) 18 
microdrepanocitosi (Beta-S) 
Blackfan-Diamond Anemia 
Dyserithropoietic Anaemia type I (CDA I) 
Sickle cell anaemia 
Piruvate-Kinase deficiency 
IRIDA iron-refractory iron deficiency anemia 
Fanconi's Anaemia 
Dyserithropoietic Anaemia type II (CDA II) 
Hyporegenerative anemia 
Megaloblastic Anemia 
Piruvate-Kinase deficiency Splenectomized 
Hemoglobinopaty (Taybe) + α-thalassemia 
Hemoglobinopaty hyperunstable (Hb Cagliari) 
Hemoglobinopaty Koln 
Acute Lymphoblastic Leukemia (ALL) 
Pyropoikilocytosis 
Evans's Syndrome 
Diagnosticn
NON BETA (control) 184 
HbH (alfa thalasemia intermedia) 129 
Alfa Thalassemia Heterozygous 48 
Beta Thalassemia Trait (study group) 30 
Hereditary Spherocytosis 28 
Iron Deficiency Anaemia 22 
Thalassemia intermedia (Beta-Thal Interm) 18 
microdrepanocitosi (Beta-S) 
Blackfan-Diamond Anemia 
Dyserithropoietic Anaemia type I (CDA I) 
Sickle cell anaemia 
Piruvate-Kinase deficiency 
IRIDA iron-refractory iron deficiency anemia 
Fanconi's Anaemia 
Dyserithropoietic Anaemia type II (CDA II) 
Hyporegenerative anemia 
Megaloblastic Anemia 
Piruvate-Kinase deficiency Splenectomized 
Hemoglobinopaty (Taybe) + α-thalassemia 
Hemoglobinopaty hyperunstable (Hb Cagliari) 
Hemoglobinopaty Koln 
Acute Lymphoblastic Leukemia (ALL) 
Pyropoikilocytosis 
Evans's Syndrome 
Results:

Using ROC analysis, the best parameters differentiating the Beta Thalassemia Trait from the normals were: MCV (AUC 1. 000), MRV (AUC 0. 999), @MAF(AUC 0. 999), @MCNRET (AUC 0. 997), RDW (AUC 0. 957), HGB (AUC 0. 915), RBC(AUC 0. 912).

Using ROC analysis, the best parameters differentiating the Beta Thalassemia Trait from other anemias (excluding normals) were: RDW-SD (AUC 0. 937), DF Eng-Fra (AUC 0. 779), RDW (AUC 0. 766), RBC (AUC 0. 734)

Disclosures:

Simon-Lopez:Beckman Coulter: @LHD, @MAF, @RSF, @LHD, @MAF, @RSF Patents & Royalties, Employment. Di Gaetano:Instrumentation Laboratory spa: Work for a distributor of Beckman Coulter Instruments in Italy Other. Galanello:Ferrokin: Research Funding; Apopharma: Research Funding, Speakers Bureau; Novartis: Research Funding, Speakers Bureau.

Author notes

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Asterisk with author names denotes non-ASH members.