Abstract
To identify known drugs with previously unrecognized anticancer activity, we compiled and screened a library of such compounds to identify agents cytotoxic to leukemia cells. From these screens, we identified ivermectin, a derivative of avermectin B1 that is licensed for the treatment of the parasitic infections, strongyloidiasis and onchocerciasis, but is also effective against other worm infestations. As a potential antileukemic agent, ivermectin induced cell death at low micromolar concentrations in acute myeloid leukemia cell lines and primary patient samples preferentially over normal hematopoietic cells. Ivermectin also delayed tumor growth in 3 independent mouse models of leukemia at concentrations that appear pharmacologically achievable. As an antiparasitic, ivermectin binds and activates chloride ion channels in nematodes, so we tested the effects of ivermectin on chloride flux in leukemia cells. Ivermectin increased intracellular chloride ion concentrations and cell size in leukemia cells. Chloride influx was accompanied by plasma membrane hyperpolarization, but did not change mitochondrial membrane potential. Ivermectin also increased reactive oxygen species generation that was functionally important for ivermectin-induced cell death. Finally, ivermectin synergized with cytarabine and daunorubicin that also increase reactive oxygen species production. Thus, given its known toxicology and pharmacology, ivermectin could be rapidly advanced into clinical trial for leukemia.
Introduction
Antimicrobials with previously unrecognized anticancer activity can be rapidly repositioned for this new indication given their extensive prior pharmacology and toxicology testing. For example, the broad spectrum antiviral ribavirin was found to suppress oncogenic transformation by disrupting the function and subcellular localization of the eukaryotic translation initiation factor eIF4E.1,2 As such, ribavirin was recently evaluated in a phase I dose escalation study in patients with relapsed or refractory M4/M5 acute myeloid leukemia (AML). In this study of 13 patients treated with ribavirin, there was 1 complete remission and 2 partial remissions. Thus, ribavirin may be efficacious for the treatment of AML.3 Likewise, the antifungal agent ketoconazole inhibits the production of androgens from the testes and adrenals in rats. Given this finding, ketoconazole was rapidly advanced into clinical trials for patients with prostate cancer where it displayed clinical efficacy in early studies.4,5
Recently, we demonstrated that the antiparasitic agent clioquinol inhibits the proteosome and induces cell death in leukemia and myeloma cells through copper-dependent and -independent mechanisms.6 Thus, our preclinical data suggest that this antiparasitic could be repurposed for the treatment of hematologic malignancies. Therefore, we initiated a phase 1 study to evaluate the dose-limiting toxicity, maximum tolerated dose, and recommended phase 2 dose of clioquinol in patients with relapsed or refractory hematologic malignancies (ClinicalTrials.gov ID NCT00963495).
Here, we used a chemical screen to identify known drugs with previously unrecognized activity against leukemia. From this screen, we identified the antiparasitic agent, ivermectin. Ivermectin is a derivative of avermectin B1 and licensed for the treatment of the parasitic infections, strongyloidiasis and onchocerciasis, as well as other worm infestations (eg, ascariasis, trichuriasis, and enterobiasis), but has not been previously tested as an anticancer agent. As part of the development of this agent as an antiparasitic agent, ivermectin was extensively evaluated for its pharmacology, safety, and toxicity in humans and animals. For example, the LD50 of oral ivermectin in mice, rats, and rabbits ranges from 10 to 50 mg/kg.7 In humans, when used to treat onchocerciasis, 100-200 μg/kg ivermectin is administered as a single dose.8 This brief and low-dose treatment is sufficient to achieve an antiparasitic effect, but higher doses and treatment beyond 1 day have been safely administered for other conditions. For example, in patients with spinal injury and resultant muscle spasticity, up to 1.6 mg/kg ivermectin was administered subcutaneously twice weekly for up to 12 weeks. In this study, no significant adverse effects were reported.9 Likewise, to evaluate the safety of oral ivermectin, healthy volunteers received 30-120 mg on days 1, 4, and 7 and then a further dose in week 3.10 Even at a dose of 120 mg (∼2 mg/kg), no serious adverse effects were noted. Finally, reports of ivermectin overdoses also support the evaluation of high doses of ivermectin in humans, as in the majority of these cases no serious adverse events were reported.11
In our current study, we demonstrated that ivermectin displayed preclinical activity against hematologic malignancies in vitro and delayed tumor growth in vivo at concentrations that appear pharmacologically achievable. Mechanistically, ivermectin induced chloride influx, membrane hyperpolarization, and generated reactive oxygen species (ROS). Furthermore, ivermectin synergized with cytarabine and daunorubicin. Thus, given its prior safety and toxicity testing, ivermectin could be rapidly advanced into clinical trial for patients with leukemia.
Methods
Reagents
The compounds in the chemical library were purchased from Sigma-Aldrich. Annexin V–fluorescein isothiocyanate (FITC) and propidium iodide (PI) were purchased from Biovision. Indo-1 AM, 6-methoxy-N-(3-sulfopropyl) quinolinium (SPQ), carboxydichlorofluorescein diacetate (Carboxy H2DCF-DA), 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl benzimidazlycarbocyanine iodide (JC1), and bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC4(3)) were all purchased from Invitrogen Canada.
Cell lines
Human leukemia (OCI-AML2, HL60, U937, KG1a) and prostate cancer (DU145 and PPC-1) cell lines and murine leukemia (MDAY-D2) cells were maintained in RPMI 1640 medium. Medium was supplemented with 10% fetal bovine serum (FBS), 100 μg/mL penicillin, and 100 U/mL streptomycin (all from Hyclone). TEX human leukemia cells were maintained in Iscove modified Dulbecco medium (IMDM), 15% FBS, 1%, penicillin-streptomycin, 20 ng/mL SCF, and 2 ng/mL IL-3. All cells were incubated at 37°C in a humidified air atmosphere supplemented with 5% CO2.
Primary cells
Primary human AML samples were isolated from fresh bone marrow and peripheral blood samples of consenting patients and mononuclear cells fractionated by Ficoll separation. Similarly, primary normal hematopoietic mononuclear cells were obtained from healthy consenting volunteers donating peripheral blood stem cells (PBSCs) for stem cell transplantation. Primary cells were cultured at 37°C in IMDM, supplemented with 20% FBS, and appropriate antibiotics. The collection and use of human tissue for this study were approved by the University Health Network institutional review board.
Chemical screen for cytotoxic compounds
HL60, KG1a, and OCI-AML2 leukemia cells were seeded into 96-well polystyrene tissue culture plates (Nunc). After seeding, cells were treated with aliquots of the chemical library (n = 100) at increasing concentrations (3-50μM), with a final dimethyl sulfoxide (DMSO) concentration of 0.5%. Seventy-two hours after incubation, cell proliferation and viability were measured by the MTS assay. Liquid handling was performed by a Biomek FX Laboratory Automated Workstation (Beckman Coulter).
Cell viability assays
Cell growth, viability, and clonogenic growth of primary cells were measured as described in the supplemental Methods (available on the Blood Web site; see the Supplemental Materials link at the top of the online article).
Assessment of ivermectin's anticancer activity in mouse models of leukemia
MDAY-D2 murine leukemia cells, K562, and OCI-AML2 human leukemia cells (2.5 × 105) were injected subcutaneously into the flanks of sublethally irradiated (3.5 Gy) nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice (Ontario Cancer Institute, Toronto, ON). Four (OCI-AML2), 5 (MDAY-D2), or 7 (K562) days after injection, once tumors were palpable, mice were then treated daily for 10 days (K562) or treated with 8 doses over 10 days (OCI-AML2) with ivermectin (3 mg/kg) by oral gavage in water or vehicle control (n = 10 per group). MDAY-D2 mice were treated similarly, but dosage escalated from 3 (4 days) to 5 (3 days) and 6 mg/kg (3 days) as the drug was well tolerated. Tumor volume (tumor length × width2 × 0.5236) was measured 3× a week using calipers. Fourteen (MDAY-D2), 15 (OCI-AML2), or 17 (K562) days after injection of cells, mice were killed, tumors were excised, and the volume and mass of the tumors were measured.
To measure gene expression changes in vivo, OCI-AML2 cells (2.5 × 105) were injected subcutaneously into the flanks of sublethally irradiated NOD/SCID mice. Once tumors were established, mice were treated with ivermectin (7 mg/kg) or vehicle control intraperitoneally for 5 days. After treatment, mice were killed and tumors were harvested. mRNA was extracted and changes in STAT expression were measured by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Evidence of apoptosis was measured by Tunel staining and immunohistochemistry (Pathology Research Program, University Health Network, Toronto, ON).
All animal studies were carried out according to the regulations of the Canadian Council on Animal Care and with the approval of the Ontario Cancer Institute Animal Ethics Review Board.
Intracellular ion measurements
Intracellular chloride concentration was measured using a fluorescent indicator for chloride, SPQ as previously described.12 Upon binding halide ions such as chloride, SPQ is quenched, resulting in a decrease in fluorescence without a shift in wavelength. After treating OCI-AML2 (5 × 105) and DU145 (4 × 105) cells overnight with ivermectin (3-10μM), cells were incubated for 15 minutes with SPQ (5mM) at 37°C in a hypotonic solution of Hanks balanced salt solution (HBSS/H2O; 1:1) to promote the intracellular uptake of SPQ. After 15 minutes of incubation with SPQ, cells were diluted (15:1) in HBSS and centrifuged. The supernatant was removed, and cells were resuspended in 200 μL of fresh HBSS and incubated for 15 minutes at 37°C to allow recovery from the hypotonic shock. Cells were then stained with PI, and SPQ fluorescence was determined in the PI negative cells using an LSR-II flow cytometer (Becton Dickinson; excitation 351 nm, emission 485 nm). In parallel, changes in cell size were determined by measuring forward light scatter by flow cytometry. Results were analyzed with FlowJo, version 8.8 (TreeStar).
Changes in cytosolic calcium concentration were detected with the fluorescent dye, Indo-1 AM (final concentration 6μM) as previously described.13
Determination of plasma and mitochondrial membrane potential and ROS generation
Plasma and mitochondrial membrane potential were measured by staining cells with DiBAC4(3) and JC-1 (Invitrogen), respectively, as described above. Intracellular ROS were detected by staining cells with Carboxy-H2DCFDA (final concentration 10μM) and analyzing with flow cytometry as previously described14 and as described in the supplemental Methods.
Gene expression studies
OCI-AML2 leukemia cells were treated with buffer control or ivermectin (3μM) for 30 and 40 hours. After treatment, cells were harvested, total RNA was isolated, and gene expression was measured as described in the supplemental Methods.
Drug combination studies
The combination index (CI) was used to evaluate the interaction between ivermectin and cytarabine or daunorubicin as previously described.15 OCI-AML2 and U937 cells were treated with increasing concentrations of ivermectin, cytarabine, and daunorubicin. Seventy-two hours after incubation, cell viability was measured by the MTS [3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. The CalcuSyn median effect model was used to calculate the CI values and evaluate whether the combination of ivermectin with cytarabine or daunorubicin was synergistic, antagonistic, or additive. CI values of < 1 indicate synergism, CI = 1 indicate additivity, and CI > 1 indicate antagonism.16
Results
A chemical screen identifies ivermectin with potential anticancer activity
Off- and on-patent drugs with previously unrecognized anticancer activity can be rapidly repurposed for this new indication given their prior toxicology and pharmacology testing. To identify such compounds, we compiled a small chemical library (n = 100) focused on antimicrobials and metabolic regulators with wide therapeutic windows and well-understood pharmacokinetics. We treated OCI-AML2, HL60, and KG1a leukemia cell lines with aliquots of this chemical library at 5 concentrations (ranging from 3-50μM). Seventy-two hours after incubation, cell growth, and viability were measured by the MTS assay. From this screen, we identified ivermectin that reduced cell viability in all cell lines in the screen with an EC50 < 10μM. The results for the screen of OCI-AML2 cells with compounds added at a final concentration of 6μM are shown in Figure 1A.
Ivermectin is cytotoxic to malignant cell lines and primary patient samples
Having identified ivermectin in our chemical screens, we tested the effects of ivermectin on cell growth and viability in a panel of 5 leukemia cell lines. Cells were treated with increasing concentrations of ivermectin, and 72 hours after incubation, cell growth and viability were assessed by the MTS assay. Ivermectin decreased the viability of the tested leukemia cell lines with an EC50 of approximately 5μM (Figure 1B). The loss of viability was detected at 24 hours after treatment and increased in a time-dependent manner. Cell death and apoptosis were confirmed by annexin V and PI staining (Figure 1C). Cell death was caspase-dependent, as cotreatment with the pan-caspase inhibitor z-VAD-fmk abrogated cell death (supplementary Figure 1A). Furthermore, times and concentrations of ivermectin that preceded cell death induced G2 cell-cycle arrest (supplemental Figure 1B and data not shown).
Given the cytotoxicity of ivermectin toward leukemia cell lines, we compared its cytotoxicity to primary normal hematopoietic cells and AML patient samples (n = 4 intermediate risk cytogenetics, n = 1 good risk cytogenetics, and n = 1 unknown cytogenetics). Normal hematopoietic cells and patient sample cells were treated for 48 hours with increasing concentrations of ivermectin. After incubation, cell viability was measured by annexin V and PI staining. Ivermectin was cytotoxic to AML patient samples at low micromolar concentrations. In contrast, it did not induce cell death in the PBSCs at concentrations up to 20μM (Figure 1C). However, when gating on the CD34+ cells from one PBSC sample, ivermectin induced cell death with an IC50 of 10.5 ± 0.6μM. Thus, ivermectin induced cell death in primary AML cells preferentially over normal cells, but the therapeutic window over normal stem cells may be narrow.
Ivermectin was also evaluated in clonogenic assays in primary normal hematopoietic and AML cells. Ivermectin (6μM) had minimal effects on the clonogenic growth of normal hematopoetic cells (n = 3) with < 15% reduction in clonogenic growth. In contrast, ivermectin reduced clonogenic growth by ≥ 40% in 3 of 6 primary AML samples (Figure 1D). Similar effects were noted when primary cells were directly plated into clonogenic assays with ivermectin (supplemental Figure 2).
Ivermectin delays tumor growth in mouse models of leukemia
Given the effects of ivermectin as a potential antileukemic agent, we evaluated ivermectin in mouse models of leukemia. Human leukemia (OCI-AML2 and K562) and murine leukemia (MDAY-D2) cells were injected subcutaneously into the flank of NOD/SCID mice. Four (OCI-AML2), 5 (MDAY-D2), or 7 (K562) days after injection, once tumors were palpable, mice were treated with ivermectin (3 mg/kg) by oral gavage in water or vehicle control (n = 10 per group) for 10 days (K562) or 8 doses over 10 days (OCI-AML2). MDAY-D2 mice (n = 10 per group) were treated similarly, but with escalating doses (3 mg/kg for 4 days, 5 mg/kg for 3 days, and then 6 mg/kg for 3 days) as the drug was well tolerated. Tumor volume and mass were measured over time. Compared with buffer control, oral ivermectin significantly (P < .05) decreased tumor mass and volume in all 3 models (Figure 2) by up to 70% without any gross organ toxicity. In an OCI-AML2 xenograft, we showed that ivermectin increased apoptosis in the subcutaneous tumor as measured by Tunel staining (supplemental Figure 3). Of note, a dose of 3 mg/kg in mice translates to a dose of 0.24 mg/kg in humans, based on scaling of body weight and surface area, and appears readily achievable based on prior studies.9,11 Thus, the activity in the xenograft studies and the in vitro studies above suggests that a therapeutic window may be achievable.
Ivermectin induces intracellular chloride flux, increase in cell size, and hyperpolarization of the plasma membrane
As an antiparasitic agent, ivermectin activates chloride channels in nematodes, causing an influx of chloride ions into the nematode's cells.17 Thus, we investigated chloride flux after ivermectin treatment in OCI-AML2 leukemia cells where ivermectin induced cell death after 24 hours of treatment and DU145 prostate cancer cells that were more resistant to ivermectin-induced cell death (Figure 3A). OCI-AML2 and DU145 cells were treated with 10μM ivermectin for 2 hours, and levels of intracellular chloride were measured by staining cells with the fluorescent dye SPQ that is quenched at high chloride ion concentrations. In OCI-AML 2 cells, ivermectin decreased SPQ fluorescence, consistent with an increase in levels of intracellular chloride at concentrations that induced cell death, but at times that preceded cell death (Figure 3B and data not shown). In contrast, chloride influx was not observed in DU145 cells that were resistant to 10μM ivermectin (Figure 3C and data not shown).
Chloride influx can increase cell size. Therefore, we measured changes in cell size in parallel to measuring changes in chloride flux. As measured by flow cytometry, after 2 hours of treatment, ivermectin caused an increase in cell size in OCI-AML2 but not in the resistant DU145 cells, consistent with its effects on chloride influx (Figure 3D-E).
In nematodes, increases in intracellular chloride after ivermectin treatment cause membrane hyperpolarization. Therefore, we evaluated the effects of ivermectin on plasma and mitochondrial membrane polarization in leukemia cells. OCI-AML2, U937, and TEX leukemia cells sensitive to ivermectin-induced death, a primary AML patient sample, DU145 and PPC-1 prostate cancer cells, and primary normal hematopoietic cells were treated with increasing concentrations of ivermectin. At increasing times after incubation, plasma membrane potential was measured by staining cells with DiBAC4(3) and flow cytometric analysis. In OCI-AML2 cells, treatment with ivermectin induced membrane hyperpolarization in a dose-dependent manner (Figure 4A) and as early as after 1 hour of treatment (Figure 4B), consistent with the influx of intracellular chloride and the effects observed in nematodes. Likewise, U937 and TEX leukemia cells, as well as primary AML cells sensitive to ivermectin-induced death, also demonstrated plasma membrane hyperpolarization after ivermectin treatment (Figure 4C). In contrast, DU145 and PPC-1 cells, as well as primary normal hematopoietic cells that were more resistant to ivermectin, did not show changes in their plasma membrane potential when treated with up to 6μM ivermectin for up to 24 hours (Figure 4D).
To determine whether the plasma membrane hyperpolarization observed after ivermectin treatment was related to increased chloride ion flux, we measured plasma membrane polarization after treating cells with ivermectin in buffers with and without chloride. OCI-AML2 cells were treated for 5 hours with ivermectin in a chloride-replete buffer or a chloride-free buffer, where sodium and potassium chloride were replaced with equimolar gluconate salts of sodium and potassium. When added to cells in the chloride-replete buffer, ivermectin induced plasma membrane hyperpolarization similar to cells treated in RPMI medium. However, when added to cells in chloride-free buffer, ivermectin caused plasma membrane depolarization (Figure 4E). Thus, the effects of ivermectin on plasma membrane polarization appear to be related to increased chloride flux.
Ivermectin increases intracellular calcium but is not functionally relevant in leukemia cells
Plasma membrane hyperpolarization can lead to calcium influx.18 Therefore, we tested the effects of ivermectin on calcium influx in leukemia cells. OCI-AML2 cells were treated with ivermectin and the concentration of intracellular calcium was measured by staining cells with the ratiometric dye, Indo-1 AM. As a positive control, cells were treated with digoxin, which is known to increase intracellular calcium.19,20 Similar to the effects of digoxin, ivermectin increased intracellular calcium (supplemental Figure 4A-B). However, the increase in intracellular calcium did not appear sufficient to explain the cytotoxicity of ivermectin, because chelation of intra- and extracellular calcium with BAPTA-AM and EDTA (ethylenediaminetetraacetic acid), respectively, did not inhibit ivermectin-induced cell death (data not shown).
Ivermectin increases intracellular ROS
Manganese chloride, cobalt chloride, and mercuric chloride can lead to generation of ROS.21-23 Therefore, we tested whether ivermectin increased ROS production in leukemia cells due to the observed chloride influx. OCI-AML2 cells were treated with ivermectin at increasing concentrations and times of incubation. After treatment, levels of intracellular ROS were measured by staining cells with Carboxy-H2DCFDA and flow cytometry. Treatment with ivermectin increased ROS production at times and concentrations that coincided with plasma membrane hyperpolarization (Figure 5A-B). Likewise, U937 and TEX leukemia cells that were sensitive to ivermectin-induced death demonstrated increased ROS generation 2 hours after ivermectin treatment (Figure 5C). In contrast, DU145 and PPC-1 cells that were more resistant to ivermectin did not show changes in ROS generation. Likewise, primary AML cells, but not normal hematopoietic cells, demonstrated increased ROS generation after ivermectin treatment (Figure 5C).
To determine whether the increased ROS production was functionally important for ivermectin-induced cell death, cells were treated simultaneously with ivermectin along with the free radical scavenger N-acetyl-L-cysteine (NAC). NAC abrogated ivermectin-induced cell death consistent with a mechanism of cell death related to ROS production and in keeping with its effects on plasma membrane hyperpolarization and chloride influx (Figure 5D).
Changes in ROS production are indicative of a biologic response to ivermectin, but are very difficult to measure in the context of a clinical trial. Therefore, to identify alterations in gene expression that are a result of ROS production and could be used as biomarkers in the context of a clinical trial, we undertook gene expression profiling analysis (using Affymetrix HG U133 Plus 2.0 arrays) of RNA derived from OCI-AML2 cells treated with ivermectin for 30 and 40 hours (supplemental Table 1). One hundred fifty genes were deregulated > 4-fold at both time points (33 underexpressed; 117 overexpressed), compared with control. Among these genes dysregulated were STAT1, which has been associated with increased ROS generation24-26 and the STAT1 downstream targets, IFIT3, OAS1, and TRIM22. We validated the up-regulation of STAT1 and target genes IFIT3, OAS1, and TRIM22 after ivermectin treatment by qRT-PCR, (Figure 6A). Likewise, U937 and HL60 leukemia cells that were sensitive to ivermectin-induced death also demonstrated increased STAT1 mRNA. In contrast, DU145 and PPC-1 cells that were more resistant to ivermectin did not show changes in STAT1 expression (Figure 6B). We also evaluated changes in STAT1 expression in tumors from a leukemia xenograft model. Mice with OCI-AML2 subcutaneous xenografts were treated with ivermectin for 5 days. After treatment, tumors were harvested, mRNA was extracted, and STAT1 expression was measured by qRT-PCR. STAT1 mRNA was increased in 2 of 3 tested tumors from mice treated with ivermectin, compared with STAT1 mRNA expression from tumors harvested from mice treated with vehicle control (Figure 6C). We also demonstrated that changes in STAT1 genes were secondary to ROS production as pretreatment with NAC blocked their up-regulation (Figure 6D).
Of note, we also compared our array dataset to a ROS gene signature reported by Tothova et al.27 Of the 55 genes in the Tothova signature, two-thirds were present in our dataset. Of these 36 genes, 55% (20 genes) were found to be differentially regulated on ivermectin treatment (fold-change of 1.25 up or down, compared with the untreated control sample). Thus, ivermectin appears to induce genetic changes consistent with ROS induction.
Ivermectin synergizes with cytarabine and daunorubicin
Cytarabine and daunorubicin increase ROS production through mechanisms related to DNA damage (Figure 7A-B) and are used clinically in the treatment of AML.28,29 Therefore, we evaluated the effects of the combination of ivermectin with cytarabine and daunorubicin on cell viability. OCI-AML2 and U937 cells were treated with increasing concentrations of ivermectin alone and in combination with cytarabine and daunorubicin. Cell growth and viability were measured 72 hours after incubation using the MTS assay. Data were analyzed by the CalcuSyn median effect model, where the CI indicates synergism (CI < 0.9), additivity (CI = 0.9-1.1) or antagonism (CI > 1.1). In both OCI-AML2 and U937 leukemia cells, the combination of ivermectin and cytarabine demonstrated strong synergism, with CI values at the ED25, ED50, and ED75 of 0.51, 0.58, and 0.65, respectively, in OCI AML2 cells and ED25, ED50, and ED75 of 0.55, 0.71, and 0.91 in U937 cells (Figure 7C). Likewise, in OCI-AML2 cells, the combination of ivermectin and daunorubicin was also synergistic, with CI values at the ED25, ED50, and ED75 of 0.48, 0.51, and 0.54, respectively. In contrast, the combination of ivermectin and daunorubicin was closer to the additive in U937, with CI values at the ED25, ED50, and ED75 of 1.1, 0.98, and 0.85, respectively (Figure 7D).
We also tested the combination of ivermectin and cytarabine in normal hematopoeitic cells. In contrast to the effects observed in the leukemia cell lines, ivermectin did not enhance the cytotoxicity of cytarabine in normal cells (Figure 7E).
Drug sequencing can affect the activity of drug combinations. Therefore, we tested the effect of drug sequencing on the synergism between ivermectin and cytarabine or daunorubicn. In OCI-AML2 and U937 cells, the combination of ivermectin and cytarabine remained synergistic, regardless of whether the ivermectin was given with, before, or after the addition of cytarabine (Figure 7F). In contrast, in OCI-AML2 cells, the combination of ivermectin was synergistic when given before or simultaneously with daunorubicin. However, the effects of the combination were additive when the ivermectin was given after the addition of daunorubicin. (Figure 7F)
We also evaluated the combination of ivermectin with the anthelmintic agent, albendazole, as this agent synergized with ivermectin in the treatment of nematodes.30,31 In contrast to the synergy observed with cytarabine and daunorubicin, albendazole antagonized the antileukemic effects of ivermectin, with CI values at the ED25, ED50, and ED75 of 1.59, 1.09, and 0.89, respectively (data not shown).
Discussion
To identify known drugs with previously unrecognized antileukemia activity, we compiled and screened a library of off- and on-patent drugs for compounds cytotoxic to leukemia cells. From this screen, we identified the antiparasitic agent, ivermectin, which induced cell death in leukemia cell lines at low micromolar concentrations and delayed tumor growth in mouse models of leukemia.
As part of its development as an antiparasitic agent, the pharmacology and toxicology of ivermectin have been studied extensively in humans and animals. Humans treated for onchocerciasis typically receive a single dose of 100-200 μg/kg ivermectin to eradicate the parasite. In such patients, plasma concentration of 52.0 ng/mL have been achieved in 5.2 hours, with an area under the curve over 48 hours of 2852 ng • h/mL.32 Similar pharmacokinetics have been reported in healthy male volunteers receiving a 14-mg capsule of radiolabeled ivermectin. In these subjects, the mean Tmax was 6 hours, with a half-life of 11.8 hours.10 These doses of ivermectin produce plasma levels that are likely lower than the concentrations required to induce an antileukemic effect and may explain why antitumor effects of ivermectin have not been previously reported in patients receiving standard doses of this drug for the treatment of onchocerciasis. However, higher concentrations of ivermectin that may possess antitumor activity have been well tolerated in both humans and animals. For example, the LD50 of oral ivermectin is approximately 28-30 mg/kg in mice, 80 mg/kg in dogs, and above 24 mg/kg in monkeys.7,32 Humans with spinal injury and muscle spasticity have been treated with up to 1.6 mg/kg of ivermectin subcutaneously twice weekly for up to 12 weeks without toxicity.9 In addition, reports of ivermectin overdoses also support the potential wide therapeutic window of this drug. For example, an individual who self-administered 6 g of veterinary ivermectin 30-50 times over the course of 1 year had no evidence of toxicity from the ivermectin.11 Multiple other ingestion events have also been reported, particularly in pediatric subjects who accidentally consumed veterinary ivermectin kept in the household for the family dog. In the majority of these, no serious adverse events were reported.9,11
Although no prior clinical studies have directly evaluated ivermectin as an anticancer agent, a case report suggests that ivermectin may have activity in the treatment of leukemia.33 An adult male with T-cell leukemia/lymphoma presented with a generalized pruritic, erythrodermic rash with areas of hyperkeratosis and was diagnosed with scabies. He received 200 μg/kg ivermectin on days 1 and 10 with complete resolution of the rash. While not a focus of the paper, it is possible that a component of the patient's rash may have been due to leukemia and this rash responded to ivermectin. Moreover, the leukemia cells in the peripheral blood were controlled while receiving ivermectin
Our studies suggest that ivermectin induces cell death through a mechanism related to its known function as an activator of chloride channels. As an antiparasitic agent, ivermectin activates glutamate-gated chloride channels unique to invertebrates. However, at higher concentrations, ivermectin also activates mammalian chloride channels.7 Mammalian chloride channels broadly fall into 5 classes, based on their regulation: cystic fibrosis transmembrane conductance regulator, which is activated by cyclic AMP dependent phosphorylation; calcium-activated chloride channels; voltage-gated chloride channels (ClCs); ligand-gated chloride channels (γ-aminobutyric acid and glycine-activated); and volume-regulated chloride channels. These channels act in heteromeric complexes dependent upon cell type, with many possible permutations and combinations of the subunits.34 Currently, it is unclear which mammalian chloride channels are being activated by ivermectin, but the complexity of their organization makes it difficult to identify single “target” channels for ivermectin activity using standard genetic experiments.
The short-term cytotoxicity studies and the in vivo experiments support a therapeutic window for ivermectin as an antileukemia agent, but the difference between normal CD34+ cells and malignant cells was narrower, as were the differences in the clonogenic growth assays. However, it is important to note that results of these assays do not always predict clinical toxicity. For example, cytarabine and amsacrine are chemotherapeutic agents routinely used in the treatment of AML, but show little or no selectivity for malignant cells over normal cells in colony formation assays.35,36 In addition, we demonstrated that oral ivermectin delayed tumor growth in 3 mouse models of leukemia without untoward toxicity, supporting a therapeutic window. Finally, toxicology studies with ivermectin in animals and humans did not report hematologic toxicity. Nonetheless, the small differential sensitivity between primary AML and normal hematopoietic cells raises concerns about the potential hematologic toxicity, and its safety will have to be carefully evaluated in phase 1 clinical trials.
The basis for the therapeutic window after invermectin treatment is likely multifactorial. Chloride channels are often increased on the surface of malignant cells, compared with normal cells, potentially making them more sensitive to alterations in chloride flux by ivermectin. For example, compared with normal neutrophils, HL60 cells overexpress the ClC-5 chloride channel that is normally expressed in renal cells.37 In support of this mechanism, we observed less chloride flux in cells more resistant to ivermectin. Alterations in intracellular chloride concentrations also affect basic homeostatic parameters, such as intracellular Ca2+ levels, pH, and cell volume,38 and alteration of these parameters can induce apoptosis.39 Finally, the therapeutic window with ivermectin treatment may reflect differences in sensitivity to ROS generation. Ivermectin increased ROS generation that appeared functionally important for its cytotoxicity, and previous studies support a mechanism of ROS generation related to increased chloride influx.22,40-42 Previous studies have also demonstrated that malignant cells have higher basal levels of ROS and are less tolerant of ROS-inducing agents, compared with normal cells.43,44 Future studies will help clarify the basis of the therapeutic window as well as identify subgroups of patients most likely to respond to this therapy.
Cytarabine and daunorubicin, which are used in the treatment of AML, induce ROS generation through a mechanism linked to DNA damage and thus a mechanism distinct from ivermectin. Consequently, we evaluated the combination of these drugs with ivermectin and demonstrated synergy with both of these drugs. Therefore, ivermectin could be evaluated in combination with these agents to enhance the efficacy of standard therapy for AML.
In summary, we have shown that ivermectin induces cell death in leukemia cells via chloride influx, membrane hyperpolarization, and increasing levels of intracellular ROS. Given its prior safety record in humans and animals coupled with its preclinical efficacy in leukemia, a phase 1 clinical trial could be conducted to determine the tolerance and biologic activity of oral ivermectin in these patients.
The online version of this article contains a data supplement.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked “advertisement” in accordance with 18 USC section 1734.
Acknowledgments
A.D.S. is a Leukemia & Lymphoma Society Scholar in Clinical Research. M.A.S. is a Canadian Institutes of Health Research postdoctoral fellow, and holds a postdoctoral research fellowship from the Multiple Myeloma Research Foundation and Millennium Pharmaceuticals. M.S. is a Canadian Institutes of Health Research Banting and Best Canada Graduate Scholar.
Authorship
Contribution: S.S. designed research, analyzed data, performed research, and wrote the paper; M.S. designed research, analyzed data, and performed research; M.A.S. designed research, analyzed data, and performed research; R.H. performed research and analyzed data; M.G. performed research and analyzed data; X.W. performed research and analyzed data; S.B.F. performed research and analyzed data; H.S. performed research and analyzed data; T.E.W. designed research, analyzed data, and performed research; R.W. performed research and analyzed data; M.D.M. contributed critical reagents and analyzed data; R.A.B. designed research and supervised research,; A.D. designed research, preformed research, and analyzed data; J.W. and S.O.K. supervised research; A.D.S. designed research, analyzed data, supervised research, and wrote the manuscript; and all authors reviewed and edited the paper.
Conflict-of-interest disclosure: The authors declare no competing financial interests.
Correspondence: Aaron D. Schimmer, Princess Margaret Hospital, Ontario Cancer Institute, 610 University Ave, Toronto, ON, Canada, M5G 2M9; e-mail: [email protected].
References
Author notes
S.S., M.S., and M.A.S. contributed equally to this work.
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