Murine bone marrow (BM) contains a mobile population of CXCR4+SSEA-1+Sca-1+lin−CD45− very small embryonic like (VSEL) stem cells (
direct immunofluorescence analysis.
To perform these studies, first the mRNA was extracted from circulating PB mononuclear cells (MNC) and the expression of pluripotent (e.g., Oct-4, Nanog) and neural specific markers (GFAP, Nestin, β-III-tubulin, Olig1, Olig) was evaluated by RQ-PCR. Next, the circulating PB MNC were analyzed by FACS for the presence of cells that express CXCR4+, CD133+ and CD34+ antigens. Finally, we measured the serum concentration of SDF-1 by ELISA. We found in stroke patients
an increase in total number of circulating CXCR4+D133+CD34+ cells,
∼ × 8 increase in expression of mRNA for Oct-4 and Nanog in circulating PB MNC that was subsequently confirmed by immunofluorescence staining for a presence of CXCR4+Oct-4+ cells, and
increase in serum concentration of SDF-1.
Additionally, we found a positive correlation between the extensiveness of a stroke and the number of CXCR4+ VSEL circulating in PB. We conclude that the stroke triggers the mobilization of CXCR4+ VSEL. We hypothesize that these cells could have a prognostic value in stroke patients as well as use for regeneration of neural tissues. Currently, we are testing this hypothesis in the murine model of stroke.
Disclosure: No relevant conflicts of interest to declare.