Resistance to anti-leukemic agents in childhood ALL has been strongly associated with prognosis. The aim of this study was to analyze the expression profile of genes related to resistance/sensibility of 4 anti-leukemic agents and their correlations with age, diagnosis WBC count, risk group classification, molecular cytogenetics, induction treatment response and event-free survival (EFS) in ALL children. Were analyzed the expression profile of 19 genes related to resistance/sensibility to vincristine (RPRP2, CD44, TCF5, KCNN1, TIF1), prednisolo ne (F8A, CDK2AP1, BLVRB, CD69), daunorubicin (MAP3K12, SHOCK2, PDCH9, EGF1, KCNN4) and L-asparaginase (GPR56, MAN1, SCGF, IGFBP7, GATA3) by real-time quantitative PCR, using SYBR Green approach and melting curve analysis, with GUSB as housekeeping gene. Relative quantification was done using the 2-DDCT method (efficiencies from 1.9 to 2.1 to all the analyzed genes). These genes were chosen according to those described on microarray analysis by Holleman et al. (
Supported by FAPESP and FAEPA
Disclosure: No relevant conflicts of interest to declare.