Abstract

Background : Patients with sickle cell anemia have a higher potential for oxidative damage due to chronic redox imbalance in their red blood cells that often leads to hemolysis, endothelial injury and recurrent vaso-occlusive episodes. In this study, we evaluated plasma levels of the anti-oxidant vitamins A, C,& E and serum level of Zinc along with serum level of Copper (pro-oxidant ).

Patients and methods: 25 adult patients with documented severe sickle cell anemia and frequent painful episodes (12 males & 13 females, aged 29.27 +/− 12.94 years) and 25 matched normal controls were studied. Plasma levels of Vitamins A, C, & E were measured by HPLC technique and serum levels of zinc & copper were measured by atomic absorption spectrometry.

Results: (Table ): Conclusion These results indicate that levels of Vitamines A, C, and E and Zinc are significantly low in patients with sickle cell anemia in comparison with matched normal controls. Besides, Copper level, which is a well known pro-oxidant, is significantly higher in sickle cell anemia patients. Therefore, supplementing sickle cell anemia patients with anti-oxidant vitamins and trace elements may contribute to the amilioration of symptoms and severity of sickle cell anemia

Levels of Vitamine A, C, & E , Zinc & Copper in patients with Sickle cell anemia and normal controls

Tested Items SCS patients Control group P-value 
Results are shown as mean +/− standard deviation., SCA = Sickle cell anemia 
Vitamin A level mcg/l 0.13 +/− 0.03 0.33 +/−0.05 <0.0001 
Vitamin C level mg/l 9.00 +/− 3.43 20.32 +/−3.74 <0.0001 
Vitamin E level, mcg/l 1.95 +/− .0.69 4.30 +/−0.80 <0.0001 
Zinc level mmol/l 9.00 +/− 1.89 11.81 +/−1.93 <0.0001 
Copper level mmol/l 24.58 +/− 4.87 18.42 +/−3.06 <0.0001 
Tested Items SCS patients Control group P-value 
Results are shown as mean +/− standard deviation., SCA = Sickle cell anemia 
Vitamin A level mcg/l 0.13 +/− 0.03 0.33 +/−0.05 <0.0001 
Vitamin C level mg/l 9.00 +/− 3.43 20.32 +/−3.74 <0.0001 
Vitamin E level, mcg/l 1.95 +/− .0.69 4.30 +/−0.80 <0.0001 
Zinc level mmol/l 9.00 +/− 1.89 11.81 +/−1.93 <0.0001 
Copper level mmol/l 24.58 +/− 4.87 18.42 +/−3.06 <0.0001 

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