WILLIFORD EPPES, ETHEL LUDOVIC; Methods: Demonstration of the "L. E." Cell without Use of Anticoagulants. Blood 1951; 6 (5): 466–469. doi: https://doi.org/10.1182/blood.V6.5.466.466
"L. E." cells and rosettes were demonstrated in the blood of patients with systemic lupus erythematosus without the use of anticoagulants. Concentration technics using defibrinated blood and unmodified blood in silicone coated tubes were employed.
