Abstract
Decreased p27Kip1 levels are a poor prognostic factor in many malignancies, and can occur through up-regulation of SCFSkp2 E3 ligase function, resulting in enhanced p27 ubiquitination and proteasome-mediated degradation. While proteasome inhibitors stabilize p27Kip1, agents inhibiting SCFSkp2 may represent more directly targeted drugs with the promise of enhanced efficacy and reduced toxicity. Using high-throughput screening, we identified Compound A (CpdA), which interfered with SCFSkp2 ligase function in vitro, and induced specific accumulation of p21 and other SCFSkp2 substrates in cells without activating a heat-shock protein response. CpdA prevented incorporation of Skp2 into the SCFSkp2 ligase, and induced G1/S cell-cycle arrest as well as SCFSkp2- and p27-dependent cell killing. This programmed cell death was caspase-independent, and instead occurred through activation of autophagy. In models of multiple myeloma, CpdA overcame resistance to dexamethasone, doxorubicin, and melphalan, as well as to bortezomib, and also acted synergistically with this proteasome inhibitor. Importantly, CpdA was active against patient-derived plasma cells and both myeloid and lymphoblastoid leukemia blasts, and showed preferential activity against neoplastic cells while relatively sparing other marrow components. These findings provide a rational framework for further development of SCFSkp2 inhibitors as a novel class of antitumor agents.
Introduction
The proteasome inhibitor bortezomib (VELCADE, Millennium Pharmaceuticals, Cambridge, MA) has demonstrated significant activity both as a single agent1,2 and in combination with other drugs3 against multiple myeloma (MM), validating the ubiquitin-proteasome pathway as a target for cancer therapy. Its broad impact on cellular proteolysis may also have deleterious effects, however, including induction of antiapoptotic heat-shock proteins (HSPs) at the molecular level, and peripheral neuropathy at the clinical level.4,5 One approach that may overcome these drawbacks would be to target E3 ligases, which are responsible for transfer of ubiquitin moieties to target proteins prior to proteasomal degradation. Since each E3 ligase serves a small subset of protein clients, this would selectively stabilize only those specific proteins, thus minimizing unwanted effects on other targets.6 SCF complex ligases are the largest family of E3 ligases, and consist of 4 components, including S-phase kinase-associated protein-1 (Skp1), Cullin-1 (Cul1), regulator of cullins-1 (Roc1), and a variable F-box protein.7 A recent crystal structure of the SCF containing the F-box protein Skp2 (SCFSkp2) revealed that Cul1 served as a scaffold binding a Skp1/Skp2 complex at its N-terminus to recruit substrates, while Roc1 bound at the C-terminus to recruit E2 ubiquitin–conjugating enzymes.8,9 Since the F-box protein determines the specificity of SCF ligases, it represents a target that could provide the greatest potential selectivity
Incorporation of Skp2 into an SCF complex confers the ability to induce ubiquitination of several targets, including p27Kip1, p21Cip1, and p57Kip2. As a critical cell-cycle regulator, p27 arrests cell division and inhibits G1/S transition, and cellular p27Kip1 levels are largely modulated through the ubiquitin-proteasome pathway. After phosphorylation at Thr187, p27 is recruited to SCFSkp2 in the nucleus to be polyubiquitinated for degradation through the 26S proteasome.10-14 Mutation or silencing of p27 in human cancers is extremely rare,15 but loss of p27Kip1 protein is a common event due to enhanced proteolysis, and has been associated with an aggressive phenotype and a poor prognosis in a variety of malignancies.16-21 In multiple myeloma, clinical studies have shown that patients with low p27 expression had a significantly shorter overall survival, while patients with high p27Kip1 expression receiving high-dose chemotherapy experienced prolonged overall survival.22-24 Also of note, an accessory protein, Cdc kinase subunit 1 (Cks1), interacts with, and increases binding between, p27Kip1 and Skp2.13 Amplification of the Cks1B locus at 1q21 is associated with a poor prognosis, transformation from monoclonal gammopathy of undetermined significance to MM, and progression of plasma cell leukemia.23,25 In acute myeloid leukemia (AML) patient specimens, the cytoplasmic to nuclear ratio of p27Kip1 is markedly related with the levels of Skp2 expression, and associated with prognosis and disease development.26 Finally, transient expression of p27Kip1 in retinoblastoma protein–defective SaOs-2 cells sensitized them to chemotherapeutic agents,27 while silencing Skp2 stabilized p27Kip1 and suppressed tumor proliferation.28-30 These data suggest that targeting the SCFSkp2 complex to restore p27Kip1 levels might be a rational strategy for cancer therapy.
Methods
Cell lines and patient samples
RPMI 8226 and U266 myeloma cells, and their doxorubicin-resistant (RPMI 8226/Dox-40) and melphalan-resistant (RPMI 8226/LR5, U266/LR6) counterparts, were kindly provided by Dr William Dalton (H. Lee Moffitt Cancer Center, Tampa, FL). Dexamethasone-sensitive MM1.S and dexamethasone-resistant MM1.R cells were kindly provided by Dr Steven Rosen (Northwestern University, Evanston, IL). NCI-H929 cells were obtained from the American Type Culture Collection (Manassas, VA), while OPM-2 cells were kindly provided by Dr Gerold Meinhardt (Klinikum der Universität München, Germany). Bortezomib-resistant ANBL-6 cells (ANBL-6/B7R) were generated by serial passage into increasing bortezomib concentrations, and maintained in 7 nM bortezomib. All myeloma cell lines were grown in RPMI 1640 with 100 mM l-glutamine, 10% fetal bovine serum, 1% penicillin/streptomycin, and, where applicable, 1 ng/mL of human recombinant interleukin-6 (IL-6; R&D Systems, Minneapolis, MN). HeLa cells from the University of North Carolina at Chapel Hill (UNC) Tissue Culture Core Facility were maintained in modified Eagle medium (MEM) supplemented as for RPMI 1640. HS-5/GFP bone marrow stromal cells provided by Dr William Dalton were maintained in supplemented RPMI 1640 and with 50 μg/mL hygromycin (Invitrogen, Carlsbad, CA). Wild-type, Skp2−/− knockout, and p27−/− knockout mouse embryo fibroblast (MEF) cells were provided by Dr Keiko Nakayama (Tohoku University, Miyagi, Japan) and Dr Keiichi Nakayama (Kyushu University, Fukuoka, Japan) and maintained in Dulbecco MEM (DMEM) supplemented with 100 mM l-glutamine, 10% fetal bovine serum, 1% sodium pyruvate, and 1% nonessential amino acids.
Primary patient samples were obtained under a University of North Carolina at Chapel Hill Institutional Review Board–approved protocol and with informed consent in accordance with the Declaration of Helsinki. Mononuclear cells from marrow aspirates or peripheral blood were separated using Ficoll-Paque Plus density sedimentation. CD138+ or CD34+ cells were enriched using immunomagnetic beads according to recommended protocols (Miltenyi Biotec, Auburn, CA).
Ubiquitination assay
HeLa cells treated with vehicle or Compound A (CpdA) for 16 hours were lysed in 20 mM HEPES, 5 mM KCl, 1.5 mM MgCl2, and 0.5 mM DTT. Ubiquitination assays were performed using 160 μg cellular extracts with 35S-methionine–labeled in vitro–translated p27 in 40 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 1 mM DTT, 2 μM ubiquitin aldehyde, 1 mg/mL methyl ubiquitin, 10 mM creatine phosphate, 0.1 μg/mL creatine kinase, 0.5 mM ATP, 1 μM okadaic acid, and 2 μg purified active His–cyclin E/Cdk2, with or without 800 ng Skp2/Skp1/Cul1/Roc1 complex as indicated. MM1.S cells were treated with 10 to 25 μM CpdA for 16 hours, and cell lysates were collected. Where indicated, an additional 400 ng His-Cks1 was added to the reactions, which were incubated at 30°C for 2 hours and stopped by adding sample buffer. Products were then separated by denaturing polyacrylamide gel electrophoresis (PAGE) and detected by autoradiography. Unless otherwise indicated, all chemicals were from Sigma-Aldrich (St Louis, MO).
Transient transfection
Complementary oligonucleotides targeting Skp2, 5′-AGCTTTTCCAAAAAAGGGAGTGACAAAGACTTTGTCTCTTGAACAAAGTC-TTTGTCACTCCCG-3′ and 5′-GATCCGGGAGTGACAAAGACTTTGTTCAAGAGACAAAGTCTTTGTCACTCCCTTTTTTGGAAA-3′, were synthesized by the UNC Nucleic Acid Core Facility and ligated into pSilencer 2.1-U6 puro (Ambion, Austin, TX). A negative control in pSilencer expressing a hairpin siRNA, shneg, with limited homology to any known sequences, was provided by Ambion. The hemaglutinin (HA)–tagged-Skp1/pCDNA3.0 plasmid was kindly provided by Dr Joon-Bok Yoon (Yonsei University, Seoul, South Korea). Nucleofector solution R was used for transfection of HeLa cells according to the manufacturer's protocol (Amaxa, Gaithersburg, MD).
Western blotting
Whole-cell extracts were prepared and separated by PAGE as previously described.31 The antibodies used included anti-Cks1, anti–phospho-(T187)–p27, anti-Skp1, or anti-Skp2 (Zymed Laboratories, South San Francisco, CA); anti-p21 (Abcam, Cambridge, MA); anti–phospho–HSP-27, HSP-27, HSP-70, and HSP-90 (Assay Designs, Ann Arbor, MI); anti–β-catenin, anti–poly-(ADP-ribose) polymerase (PARP), anti–caspase-3, anti–caspase-8, anti–caspase-9, anti-p27, and anti-p57 (Cell Signaling Technology, Danvers, MA); and anti–β-actin, anti–c-Jun, and anti–microtubule-associated protein light chain 3 (MAP-LC3; Santa Cruz Biotechnology, Santa Cruz, CA). For immunoprecipation, 4 μg anti-HA antibody (Santa Cruz Biotechnology) was added to 500 μg whole-cell extracts, along with 20 μL protein A/G plus beads (Santa Cruz Biotechnology), and incubated overnight. Immunoprecipitates were collected and washed in lysis buffer, boiled in sample buffer, and then subjected to Western blotting.
Growth inhibition assay
Myeloma cells were incubated with CpdA in 2-fold serial dilutions from 2 × 10−5 to 6.25 × 10−7 M. Cells from 3-day cultures were then incubated with the tetrazolium reagent WST-1 (Roche Applied Bioscience, Indianapolis, IN) for 1 to 3 hours, and absorbance was measured at 450 nm, with a reference wavelength of 650 nm. The viability of control cells treated with vehicle was arbitrarily set at 100%.
Caspase inhibition assay
RPMI 8226 cells were preincubated with vehicle or 50 μM PAN-caspase inhibitor (EMD Biosciences, San Diego, CA) for 2 hours before treatment with CpdA or bortezomib, and cell viability was determined after 24 hours as described in “Growth inhibition assay.”
Flow cytometric analysis
Myeloma cells cultured in the presence of CpdA or bortezomib were stained with annexin V–fluorescein isothiocyanate (FITC) or annexin V–phycoerythrin (PE; BioVision, Mountain View, CA) as well as the TO-PRO-3 nuclear dye (Invitrogen) for flow cytometry. RPMI 8226 cells treated with both caspase inhibitor or bafilomycin A1 (Sigma-Aldrich), and drugs were double-stained with FITC-VAD-FMK and annexin V–PE and collected for flow cytometry to detect caspase-independent annexin V staining. Propidium iodide (PI) staining was performed to measure cell-cycle progression effects as previously described.31
Adhesion of myeloma cells to bone marrow stromal cells
HS-5/GFP stromal cells seeded at 4 × 104 cells/well in 12-well plates were incubated overnight and washed with serum-free medium, and 105 MM1.S cells were added, followed by exposure to vehicle, 5 or 10 μM CpdA, or 2.5 nM bortezomib for 18 hours. MM1.S cells were detached by pipetting and stained with annexin V–PE and TO-PRO-3, and a GFP− population was analyzed by flow cytometry. For dexamethasone treatment, MM1.S cells with or without HS-5/GFP stroma were treated with vehicle or 10 μM dexamethasone in ethanol for 2 days, and analyzed as described in “Flow cytometric analepis.”
MDC staining
HeLa cells were treated with dimethyl sulfoxide (DMSO), CpdA alone, or with 200 nM bafilomycin A1 for 4 hours. Monodansylcadaverine (MDC; Sigma-Aldrich) dissolved in 1:1 DMSO/ethanol was added to a final concentration of 100 μM for 1 hour. The cells were then fixed using 4% formaldehyde in phosphate-buffered saline (PBS) and visualized by fluorescence microscopy.
Electron microscopy
Cells were harvested after treatment with DMSO, CpdA, or bortezomib, and washed with PBS. Following fixation in 2% paraformaldehyde/2.5% glutaraldehyde, pellets were rinsed and postfixed in 1% osmium tetroxide/1.25% potassium ferrocyanide. Samples were dehydrated in a graded series of ethanols, followed by propylene oxide, and infiltrated and embedded in Polybed 812 resin (Polysciences, Warrington, PA). Thick 1-μm sections were cut with a glass knife, mounted on slides, stained with toluidine blue, and viewed using a light microscope. Ultrathin 70-nm sections were taken from areas selected by light microscopy, mounted on 200 mesh copper grids, and stained with uranyl acetate and lead citrate. These were observed and photographed using a LEO EM-910 transmission electron microscope (LEO Electron Microscopy, Thornwood, NY) at an accelerating voltage of 80 kV.
Statistical analysis
The interaction between anti-MM agents was analyzed using CalcuSyn software (BioSoft, Cambridge, United Kingdom). A combination index (CI) of less than 1.0 indicates synergy, while a CI of 1.0 indicates additive activity. The Student t test was used for other statistical analyses.
Results
Identification of an inhibitor of SCFSkp2-mediated ubiquitination
To screen for compounds that could inhibit p27Kip1 ubiquitination, we prepared an in vitro–reconstituted system incorporating purified cyclin E/Cdk2, Skp2, Skp1, Cul1, Roc1, and cellular extract from vehicle-treated HeLa cells. This system ubiquitinated 35S-labeled, in vitro–transcribed and –translated p27, as measured by the appearance of a ladder of higher-molecular-weight polyubiquitinated p27Kip1 (Figure 1A lane 2). After adapting it to a high-throughput format, several libraries were screened, ultimately leading to the identification of CpdA (Figure 1B). In comparison with DMSO-treated cells, reduced in vitro p27 ubiquitination was observed with cell extracts prepared from CpdA-treated HeLa cells (Figure 1A lane 3). Addition of excess, preformed Skp2/Skp1/Cul1/Roc1 overcame inhibition by CpdA, and restored p27 ubiquitination (Figure 1A lane 6), indicating that the SCFSkp2 activity in CpdA-treated cells was limited. In order to evaluate the impact of CpdA on p27Kip1 in a cellular context, HeLa cells were arrested in S phase, exposed to either vehicle, the proteasome inhibitor MG132, or CpdA, then treated with cycloheximide and evaluated for their p27 levels. Compared with vehicle-treated controls (Figure 1C), MG132 prolonged the half-life of p27 and phospho-p27, as did CpdA (Figure 1C; Figure S1A, available on the Blood website; see the Supplemental Materials link at the top of the online article).
To evaluate the mechanism of action of CpdA, HeLa cells were transfected with a control small hairpin interfering RNA (shneg) or one inducing down-regulation of Skp2 (shSkp2; Figure 1D left panel) and then treated with vehicle or CpdA. In HeLa/shneg cells, CpdA induced the accumulation of p27, as well as other SCFSkp2 substrates, including p21 and p57 (Figure 1D right panel). However, in HeLa/shSkp2 cells, though as expected basal levels of p21, p27, and p57 were higher compared with HeLa/shneg controls, CpdA did not further increase the levels of these proteins. In order to further test the hypothesis that CpdA was affecting the SCFSkp2 complex, HeLa cells were transfected with HA-tagged Skp1 and treated with vehicle or CpdA, and then immunoprecipitation was performed (Figure 1E). Skp2 was detectable in immunoprecipitates from vehicle-treated cells, but was not visible when these had been treated with CpdA, suggesting Skp2 was being excluded from the SCF complex. A dependence on Skp2 was also supported by studies in wild-type (WT), p27−/−, and Skp2−/− MEF cells (Figure 1F). CpdA induced p21, p27, and p57 accumulation in WT cells, and p21 and p57 accumulation in p27−/− MEFs, but in Skp2−/− MEFs, CpdA was unable to affect the abundance of these proteins. Also of note, WT MEF cells were treated with increasing CpdA doses, which resulted in a dose-dependent decrease in viability (Figure 1G). Interestingly, Skp2−/− MEFs were more resistant, and even at the highest CpdA doses tested showed only a small decrease compared with vehicle-treated controls. Similarly, p27−/− MEFs were more resistant, though not to the same extent as Skp2−/− cells. Taken together, these studies suggest that this novel agent specifically targets the SCFSkp2-p27 pathway, interferes with the Skp1/Skp2 interaction to prevent SCFSkp2 E3 ligase function, and stabilizes p27.
Impact of CpdA in myeloma models
Since the ubiquitin-proteasome pathway has best been validated as a therapeutic target in MM, it was of interest to compare it with CpdA in this context. MM1.S myeloma cells were therefore treated either with vehicle or CpdA, and their extracts were used to catalyze p27 ubiquitination in vitro. As was the case for HeLa cells, CpdA-treated MM1.S cell extracts were less able to catalyze ubiquitination of p27Kip1 (Figure 2A left panel) in a dose-dependent fashion. Excess preformed Skp2/Skp1/Cul1/Roc1 complexes overcame inhibition by CpdA (Figure 2A middle panel). Since Cks1 dramatically enhances binding between SCFSkp2 and p27, promoting ubiquitination and degradation of p27,13,23,25,32 we sought to exclude the possibility that Cks1 might also serve as a CpdA target. Excess Cks1 was therefore added, but did not overcome inhibition by CpdA, and did not restore p27 ubiquitination (Figure 2A right panel).
To study the effect of CpdA on intracellular proteolysis, RPMI 8226 cells (Figure 2B) were treated with CpdA or bortezomib. CpdA increased abundance of the SCFSkp2 substrates p21Cip1, p27Kip1, and p57Kip2 (Figure 2B left panel), as did bortezomib. However, clients of other SCF E3 ligases, such as β-catenin, a target of SCFβ-TrCP, and c-Jun, a target of SCFFbw7, were affected only by bortezomib. Notably, the levels of Skp2, Cks1, Cdk2, and Skp1, components of the SCFSkp2 complex, were not altered by CpdA (Figure 2B right panel). Finally, bortezomib was seen to activate the HSP response in RPMI 8226 cells (Figure 2C), as evidenced by increased HSP-70 levels, as well as activation of HSP-27 seen as an increase in phospho–HSP-27. Consistent with its much more narrow effect on intracellular proteolysis, however, CpdA did not have any of these effects. Similar results were also obtained in MM1.S cells (Figure S1B,C). Taken together, these findings support the hypothesis that this agent, as a novel inhibitor of SCFSkp2 E3 ligase function, is much more selective than proteasome inhibitors.
Effect of CpdA on the cell cycle and apoptosis
To study the potential of SCFSkp2 as a therapeutic target for MM, a panel of cell lines were treated, and their viability was then evaluated. Inhibition of SCFSkp2 resulted in a dose-dependent decrease in viability in all cell lines tested, with 50% inhibition (IC50) values in the micromolar range (Figure 3A). Cell-cycle analysis of RPMI 8226 cells was then performed (Figure 3B), and revealed an accumulation of myeloma cells in G0/G1 phase, with a relative paucity of cells traversing through S and G2/M phases. In addition to cell-cycle arrest, CpdA activated programmed cell death (PCD) in a dose-dependent fashion (Figure 3C), as measured by an increase in positive staining with annexin V. Interestingly, while CpdA induced externalization of membrane phosphatidyl-serine residues (Figure 3C), it did not activate type I PCD with oligonucleosomal fragmentation, since flow cytometry revealed no appearance of cells with a sub-G1 DNA content (Figure 3B). Also, bortezomib concentrations that activated PCD to the same extent as did CpdA (Figure 3C) triggered cleavage of caspase-8, caspase-9, and the common effector caspase-3 (Figure 3D), as well as PARP. However, CpdA did not activate either the extrinsic or the intrinsic caspase pathways. Indeed, while survival of myeloma cells after bortezomib was enhanced by preincubation with the PAN-caspase inhibitor Z-VAD-FMK (Figure 3E) from 35% to 65% (P < .05), PAN-caspase inhibition did not protect cells from CpdA-induced cell death (Figures 3E,S1D). Finally, PAN-caspase inhibition reduced annexin V staining induced by bortezomib nearly to control, vehicle-treated levels (Figure 3F), while no protective effect was seen in regard to CpdA. Thus, CpdA reduced viability and inflicted cell-cycle arrest as well as programmed cell death, but the latter occurred in a caspase-independent manner.
Staining with annexin V can detect caspase-independent apoptosis33 occurring through type II PCD, or autophagy, and it was therefore of interest to determine if CpdA was activating this pathway. One hallmark of autophagy is the formation of vacuoles that stain with MDC, resulting in a punctate fluorescence. CpdA-treated HeLa cells exhibited this pattern (Figure 4A middle panel), which was suppressed by preincubation with bafilomycin A1, an autophagy inhibitor. Due to the small size of MM cells, analysis for MDC staining at the light microscopic level was unreliable. Using flow cytometry, therefore, we found that CpdA induced the appearance of a large population of annexin V+/caspase-3− cells (Figure 4B), while bortezomib induced appearance of annexin V+/caspase-3+ cells. Addition of bafilomycin A1 to CpdA significantly increased the viable, annexin V−/caspase-3− population, while decreasing annexin V+/caspase-3− cells. Notably, bafilomycin A1 did not inhibit the ability of bortezomib to induce apoptotic annexin V+/caspase-3− cells, and if anything decreased the proportion of annexin V+/caspase-3− cells, representing those undergoing autophagy. Another autophagy marker is cleavage of MAP-LC3, a human homolog of the yeast essential autophagy protein Atg8. MAP-LC3-I is processed from its full-length, 18-kDa form to a 16-kDa form, MAP-LC3-II, and the abundance of MAP-LC3-II reflects the levels of autophagy.34-36 Incubation of myeloma cells with CpdA enhanced LC3-II formation, indicating autophagic induction (Figures 4C,S1E), while such cleavage was not enhanced in bortezomib-treated cells. Finally, electron microscopy was used to examine the subcellular appearance of myeloma cells undergoing these treatments. Bortezomib induced the appearance of multiple hyperdense intracellular structures (Figure 4D top right panel) consistent with early chromatin condensation and fragmentation, ultimately forming apoptotic bodies. After treatment with CpdA, a different morphology was seen, with numerous double-walled membrane-enclosed cytoplasmic vacuoles. In some sections (Figure 4D bottom right panel), these vacuoles contained intracellular organelles, such as mitochondria. These findings demonstrated that CpdA exerted its antiproliferative and proapoptotic effects through activation of autophagic cell death.
Inhibition of SCFSkp2 and drug resistance of CpdA
Targeting SCFSkp2 is a possible novel approach to cancer therapy, and given its activity against myeloma it was of interest to determine its potential in overcoming drug resistance and inducing chemosensitization. Melphalan resistance was studied by comparing parental RPMI 8226 and U266 cells, with RPMI 8226/LR5 and U266/LR6 cells, respectively (Figure 5A). Both RPMI 8226 and U266 cells were sensitive to CpdA, and their melphalan-resistant counterparts were sensitive as well, with comparable IC50 values. Similar comparisons of RPMI 8226 and RPMI 8226/Dox, and MM1.S and MM1.R cells, showed that CpdA was equally active against the doxorubicin- and steroid-resistant clones, if not more so. The only clinically relevant drug targeting the ubiquitin-proteasome pathway at this time is bortezomib, and we also studied the interactions between that agent and CpdA. Notably, in bortezomib-resistant ANBL-6/B7R cells (Figure 5A), CpdA inhibited proliferation with equal potency to that in WT ANBL-6 cells. Also, when CpdA was added to bortezomib, this combination had enhanced activity against RPMI 8226 (Figure 5B). Isobologram analysis indicated CIs that were consistent with a synergistic interaction. CpdA was also synergistic with bortezomib against IL-6–dependent KAS-6/1 cells (Figure 5C). Another clinically relevant aspect of MM cells is their expression of adhesion-mediated drug resistance upon interaction with bone marrow stromal cells.37,38 We next therefore tested whether CpdA could overcome this resistance in MM1.S cells cultured with or without HS-5/GFP stromal cells, and then exposed to CpdA, bortezomib, or dexamethasone. Consistent with previous reports, coculture did protect MM1.S from dexamethasone (P < .05), but not from bortezomib-mediated apoptosis (Figure 5D). Similarly, MM1.S cell death induced by CpdA was not suppressed by stromal cells, indicating that agents targeting SCFSkp2 may overcome multiple drug resistance mechanisms, and induce chemosensitization.
Activity of CpdA against patient-derived malignant cells
To further define the potential efficacy of CpdA, we evaluated its activity against malignant cells freshly isolated from patients with various hematologic malignancies. Plasma cells were purified by CD138+ selection of bone marrow aspirates from patients with MM (Figure 6A). When they were exposed to CpdA, a dose-dependent inhibition of viability was noted in all samples tested. Unsorted bone marrow mononuclear cells were used as a control to evaluate the potential specificity of this agent. A dose-dependent decrease in viability was observed in the CD138+ neoplastic plasma cells (Figure 6B top panels). Importantly, cells that were CD138− (Figure 6B bottom panels), a fraction enriched for nonneoplastic normal bone marrow cells, were less affected. In the CD138+ cell fraction, viability decreased from 66% to 0.3% after treatment, while in CD138− cells, this decreased from 94% to 62%. Similar results were also observed in mononuclear cells from one patient with acute lymphoblastic leukemia (ALL). Viability of CD34+ leukemic blasts decreased from 37.0% to 3.19% after treatment with CpdA (Figure 6C), while in CD34− cells, this decreased from 45.0% to 42.9%.
Elevated Skp2 levels have been described to represent a poor prognostic factor in patients with AML, and it was therefore of interest to examine the activity of CpdA in such samples. Leukemic blasts from the peripheral blood of 2 patients with AML were studied, and on exposure to CpdA both were induced to undergo apoptosis (data not shown). Also, both showed increased abundance of p27Kip1 (Figure 6D), as did one myeloma patient sample, suggesting that, like bortezomib, CpdA was interfering with p27 turnover. Finally, CpdA was studied in combination with bortezomib, and while both were active in inducing cell death of leukemia blasts alone (Figure 6E), together they acted in a synergistic manner (Figure 6F).
Discussion
Targeting E3 ligases as part of cancer therapy has been proposed as a rational strategy due to the potential for enhanced specificity in comparison with other approaches to modify intracellular proteolysis, such as proteasome inhibition. Overexpression of Skp2 and decreased levels of p27 have been shown to be related to prognosis in a variety of cancers,39 while silencing of Skp2 to up-regulate p27, or overexpression of p27 itself, inhibited cancer cell proliferation and decreased cell survival.28-30,40,41 Therefore, the E3 ligase SCFSkp2 provides a potential drug target for cancer therapy, but pharmacologic inhibitors of its action have not been previously reported.
In the current work, we report the identification of a small molecule that inhibited in vitro p27 ubiquitination, and also stabilized p27 in cell-line model systems (Figures 1,2,S1). Other SCFSkp2 complex substrates, such as p21 and p57, were also stabilized, while substrates of different SCF E3 ligases were unaffected (Figures 2,S1). The narrower effect of this agent on intracellular proteolysis was demonstrated by a lack of accumulation of ubiquitin-protein conjugates (data not shown), and a lack of activation of the HSP response (Figures 2,S1). When gene expression profiles of DMSO- and CpdA-treated RPMI 8226 cells were compared, there were only 78 genes that showed an at least 2-fold change (data not shown), and since p27 was not among them, accumulation of p27 was not due to up-regulation of messenger expression. This further indicates that, unlike the proteasome inhibitor bortezomib, which had a much broader impact on gene expression profiles,4,42 CpdA had a comparatively limited effect on transcriptional activity. Mechanistic studies suggested that inhibition of p27 ubiquitination by CpdA involved direct targeting of SCFSkp2 E3 ligase function by excluding Skp2 from the SCF complex, in that immunoprecipitation failed to identify this F-box protein when Skp1 was isolated in the presence of this compound, compared with controls (Figure 1E). Since Skp2 expression itself was not affected, CpdA may act by disrupting binding between Skp1 and Skp2. MEF cell studies further indicated that CpdA-induced cell death was mainly due to its effect on the SCFSkp2-p27 pathway, since p27−/− MEFs showed only a slightly smaller degree of resistance to CpdA than Skp2−/− MEFs. Recent studies have shown that neddylation of Cul1 is an important step in assembly of this E3 ligase,43 and that cyclin D1 and the phosphatase and tensin homolog on chromosome 10 may also play roles.44 It is therefore possible that CpdA may function through other mechanisms, such as by interfering with Cul1 neddylation. Further studies will be needed, and are ongoing, to better define the pathways affected by CpdA and its homologs.
Inhibition of SCFSkp2 blocked proliferation of neoplastic cells by inducing G1/S cell-cycle arrest and apoptosis (Figure 3). Interestingly, the latter did not occur through caspase-mediated pathways, since proteolytic cleavage of caspase-8, caspase-9, and caspase-3 was not seen, and PAN-caspase inhibition did not suppress apoptosis. Instead, CpdA activated type II PCD, or autophagy, based on the appearance of MDC cell staining and annexin V+/caspase-3− cell populations, both of which were suppressed by bafilomycin A1 (Figure 4). Moreover, proteolytic processing of MAP-LC3 and electron microscopy showing the appearance of autophagic vacuoles also supported this conclusion. Up-regulation of p27 via mammalian target of rapamycin inhibition has been described to induce cell-cycle arrest at the G0/G1 phase in association with the induction of autophagy.45 Also, direct overexpression of p27 in cancer cells induced autophagy40,41 and showed a greater antitumor effect than did the overexpression of other cyclin-dependent kinase inhibitors.40 This suggests that stabilization of p27 by CpdA may be responsible for activation of type II PCD, but additional studies will be needed in this regard. The transcriptional profile of CpdA-treated cells showed up-regulation of the autophagy gene human WD-repeat protein interacting with phosphoinosides (h-WIPI-1α), a human homolog of the yeast Atg18 gene,46 which is vital for all forms of autophagy in Saccharomyces cerevisiae.47 Studies have shown that hWIPI-1 could colocalize in part with the autophagosomal marker LC3 at punctate cytoplasmic structures, and the accumulation of hWIPI-1 in cytoplasmic vesicular structures occurred during amino acid deprivation–induced autophagy.46 Thus, direct or indirect induction of WIPI49 by CpdA may also contribute to activation of type II PCD.
Autophagy is necessary for survival of cells under unfavorable conditions to recycle microorganelles, but it may also induce cell death when excessive autophagy occurs. A number of studies have reported that this pathway is activated in cancer cells in response to DNA-damaging agents and other anticancer therapies,48-50 such as arsenic trioxide, rapamycin, and tamoxifen. Combinations of autophagy-inducing agents and proteasome inhibitors have shown synergistic cell killing.51 This provided a rationale for combining CpdA with bortezomib, and indeed, CpdA and bortezomib were found to be synergistic (Figure 5), and CpdA even overcame resistance to bortezomib. The basis for this synergy may be the ability of CpdA to prevent turnover of the polyubiquitinated proteins accumulated by proteasome inhibition through the autophagic pathway, thus targeting major nonlysosomal and lysosomal protein degradation mechanisms. Alternatively, synergy may be a result of this combination's ability to activate both caspase-dependent and -independent apoptosis.
Understanding the mechanism of action of agents such as CpdA may allow us to rationally design new methods of treatment using this compound in combination with other anticancer drugs. Interestingly, CpdA did not enhance the effects of either melphalan or doxorubicin (data not shown). As a DNA cross-linker, melphalan targets S-phase DNA and delays cell-cycle progression into the G2/M phase,31 while CpdA arrests the cell cycle at G0/G1. One possible explanation for this is that SCFSkp2 inhibition prevented cells from entering S phase, and therefore protected them from DNA damage by melphalan. It is not yet clear why the combination of CpdA and doxorubicin were not synergistic in MM cells. It has been observed that overexpression of p27 in human colon cancer cells decreased their tumorigencity in nude mice, however, and the p27-overexpressing tumors were more resistant to intraveneous doxorubicin.52 Furthermore, in MCF-7 breast cancer cells, subcellular distribution of p27 was associated with cross-resistance to doxorubicin.53 These studies indicate that accumulation of p27 might have negative consequences in treatment outcomes with anthracyclines. Another recent study showed that p27 was stabilized in non-Hodgkin lymphomas when cells were adhered to bone marrow stromal cells. The accumulation of p27 was due to down-regulation of the SCFSkp2 E3 ligase pathway.38 Our study showed that although CpdA targeted the SCFSkp2-p27 pathway, the agent was able to overcome stroma-mediated drug resistance.
Finally, we were able to document activity of CpdA in neoplastic cell populations from patients with MM, ALL, and AML, with evidence of relative sparing of nonneoplastic cells (Figure 6). Of the 4 patients with myeloma examined, MM-24, MM-26, and MM-39 had 13q deletions and were bortezomib-naive, while MM-21 had clinically progressed on a bortezomib-based regimen. These studies suggest that CpdA has the potential for activity in clinically high-risk patients with myeloma, and may overcome clinical bortezomib resistance. Similarly, patient AML-004 had relapsed disease after prior induction therapy with daunorubicin, cytarabine, and etoposide, followed by consolidation with high-dose cytarabine. Given the role of p27 as a prognostic factor in patients with solid tumors as well, we have evaluated the activity of CpdA in other cell lines, and found activity against models of breast cancer, prostate cancer, and glioma, among others, again with evidence of induction of autophagy (data not shown). One limitation of this agent is its relative lack of potency, in that it requires micromolar concentrations for efficacy, limiting the ability to perform in vivo studies. We are currently evaluating stereoisomers of this compound that seem to have enhanced potency, and structure activity relationship studies may help in optimizing its structure further. These studies do provide a proof of principle, however, provide a framework for further development of agents such as CpdA that target SCFSkp2, and support their great potential to become important parts of our future chemotherapeutic armamentarium against a variety of malignancies.
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Acknowledgments
We thank Victoria J. Madden and Elena Davis for their assistance in performing electron microscopy, and Brydon Bennett for helpful comments on the manuscript.
This work was supported by a Ruth L. Kirschstein National Research Service award (to Q.C.) and a Multiple Myeloma Research Foundation fellow award (to Q.C.). R.Z.O., a Leukemia & Lymphoma Society Mansbach Foundation Scholar in Clinical Research and a Jefferson-Pilot Fellow in Academic Medicine, would also like to acknowledge support from the Leukemia & Lymphoma Society (6096-07), the National Cancer Institute (RO1 CA102278), and the Multiple Myeloma Research Foundation.
National Institutes of Health
Authorship
Contribution: Q.C. designed and performed the majority of the research and wrote the manuscript; W.X., A.L.-G., D.M., L.G.C., V.P.K., W.X., L.M.-d.P., D.R.W., and F.M. were involved in the compound screening, chemical synthesis, and ubiquitination assays; D.J.K. and P.M.V. were essential for helping with patient sample purifications; K.I.N. and K.N. provided MEF WT, p27−/−, and Skp2−/− knockout cells; and R.Z.O. supervised all the research and wrote the manuscript.
Conflict-of-interest disclosure: W.X., A.L.-G., D.M., L.G.C., V.P.K., W.X., L.M.-d.P., D.R.W., and F.M. are employees of Celgene, and receive stock options as part of their employment. All other authors declare no competing financial interests.
Correspondence: Robert Z. Orlowski, The University of Texas M. D. Anderson Cancer Center, Department of Lymphoma/Myeloma, 1515 Holcombe Blvd, Unit 429, Houston, TX 77030-4009; e-mail: [email protected].