TO THE EDITOR:

Several studies showed that skeletal muscle myosin (SkM) and cardiac myosin (CM) can support prothrombinase complex (factor [F]Xa-FVa-Ca++) activity that generates thrombin and also support downregulation of thrombin generation by activated protein C-protein S proteolysis of FVa.1-5  Those studies included data indicating an essential role for the myosin protein component of SkM and CM preparations for their prothrombinase activity. First, antimyosin antibodies (Abs) inhibit the ability of SkM and CM to support prothrombinase. Furthermore, immobilized anti-myosin Abs remove prothrombinase supporting activity from SkM and CM preparations.1,3  Second, studies showed that the SkM heavy chain peptide HC815-837 inhibits SkM-enhanced prothrombinase and directly binds FXa, which strongly implies that it inhibits protein-protein interactions involving direct binding of FXa to SkM. Notably, peptide HC815-837 does not inhibit phospholipid (PL)-enhanced prothrombinase, emphasizing its direct effect on SkM-FXa...

REFERENCES

REFERENCES
1.
Deguchi
H
,
Sinha
RK
,
Marchese
P
, et al
.
Prothrombotic skeletal muscle myosin directly enhances prothrombin activation by binding factors Xa and Va
.
Blood
.
2016
;
128
(
14
):
1870
-
1878
.
2.
Deguchi
H
,
Guo
Z
,
Hayat
M
, et al
.
Molecular interaction site on procoagulant myosin for factor Xa-dependent prothrombin activation
.
J Biol Chem
.
2019
;
294
(
41
):
15176
-
15181
.
3.
Zilberman-Rudenko
J
,
Deguchi
H
,
Shukla
M
, et al
.
Cardiac myosin promotes thrombin generation and coagulation in vitro and in vivo
.
Arterioscler Thromb Vasc Biol
.
2020
;
40
(
4
):
901
-
913
.
4.
Heeb
MJ
,
Fernández
JA
,
Yamashita
A
, et al
.
Activated protein C anticoagulant activity is enhanced by skeletal muscle myosin
.
Haematologica
.
2020
;
105
(
8
):
e424
-
e427
.
5.
Coleman
JR
,
Moore
EE
,
Zilberman-Rudenko
J
, et al
.
Cardiac and skeletal muscle myosin exert procoagulant effects
.
Shock
.
2019
;
52
(
5
):
554
-
555
.
6.
Novakovic
VA
,
Gilbert
GE
.
Procoagulant activities of skeletal and cardiac muscle myosin depend on contaminating phospholipid
.
Blood
.
2020
;
136
(
21
):
2469
-
2472
.
7.
Moody
MA
,
Liao
HX
,
Alam
SM
, et al
.
Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines
.
J Exp Med
.
2010
;
207
(
4
):
763
-
776
.
8.
Li
T
,
Aredo
B
,
Zhang
K
, et al
.
Phosphatidylserine (PS) is exposed in choroidal neovascular endothelium: PS-targeting antibodies inhibit choroidal angiogenesis in vivo and ex vivo
.
Invest Ophthalmol Vis Sci
.
2015
;
56
(
12
):
7137
-
7145
.
9.
Thomas
JM
,
Thorpe
PE
.
Protective effect of anti-phosphatidylserine antibody in a guinea pig model of advanced hemorrhagic arenavirus infection
.
Open Microbiol J
.
2017
;
11
(
1
):
303
-
315
.
10.
Shi
J
,
Gilbert
GE
.
Lactadherin inhibits enzyme complexes of blood coagulation by competing for phospholipid-binding sites
.
Blood
.
2003
;
101
(
7
):
2628
-
2636
.
11.
Blackwood
RA
,
Ernst
JD
.
Characterization of Ca2(+)-dependent phospholipid binding, vesicle aggregation and membrane fusion by annexins
.
Biochem J
.
1990
;
266
(
1
):
195
-
200
.
12.
Weingärtner
A
,
Kemmer
G
,
Müller
FD
, et al
.
Leishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment
.
PLoS One
.
2012
;
7
(
8
):
e42070
.
13.
Meers
P
,
Mealy
T
.
Calcium-dependent annexin V binding to phospholipids: stoichiometry, specificity, and the role of negative charge
.
Biochemistry
.
1993
;
32
(
43
):
11711
-
11721
.
14.
Meers
P
,
Mealy
T
.
Relationship between annexin V tryptophan exposure, calcium, and phospholipid binding
.
Biochemistry
.
1993
;
32
(
20
):
5411
-
5418
.
15.
Kamikubo
Y
,
Mendolicchio
GL
,
Zampolli
A
, et al
.
Selective factor VIII activation by the tissue factor-factor VIIa-factor Xa complex
.
Blood
.
2017
;
130
(
14
):
1661
-
1670
.
16.
Speidel
CM
,
Eisenberg
PR
,
Ruf
W
,
Edgington
TS
,
Abendschein
DR
.
Tissue factor mediates prolonged procoagulant activity on the luminal surface of balloon-injured aortas in rabbits
.
Circulation
.
1995
;
92
(
11
):
3323
-
3330
.
17.
Deguchi
H
,
Shukla
M
,
Hayat
M
,
Torkamani
A
,
Elias
DJ
,
Griffin
JH
.
Novel exomic rare variants associated with venous thrombosis
.
Br J Haematol
.
2020
;
190
(
5
):
783
-
786
.
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